A fluorescent indicator for the bioimaging of nitric oxide
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DAN-1 EE (hydrochloride)

Item No. 85070

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
4-[[(3-amino-2-naphthalenyl)amino]methyl]-benzoic acid, ethyl ester, monohydrochloride
CAS Number
1049720-51-5
Molecular Formula
C20H20N2O2 • HCl
Formula Weight
356.9
Purity
≥95%
Emission
420-450 nm
Excitation
360-380 nm
Formulation (Request formulation change)
A 10 mg/ml solution in ethanol
Solubility(Learn about Variance in Solubility)
DMF: ~30 mg/mlDMSO: ~30 mg/mlEthanol: ~30 mg/mlPBS pH 7.4: 0.5 mg/ml
λmax
251 nm
SMILES
NC1=CC2=CC=CC=C2C=C1NCC3=CC=C(C(OCC)=O)C=C3.Cl
InChi Code
InChI=1S/C20H20N2O2.ClH/c1-2-24-20(23)15-9-7-14(8-10-15)13-22-19-12-17-6-4-3-5-16(17)11-18(19)21;/h3-12,22H,2,13,21H2,1H3;1H
InChi Key
IMTHVIIBTLZVBZ-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    1 mg

    $41

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    5 mg

    $178

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    10 mg

    $312

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    50 mg

    $1,356

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    Product Description

    DAN-1 EE is a fluorescent indicator for the bioimaging of nitric oxide (NO).1 DAN-1 EE is a cell permeable derivative of DAN, a molecule which has been used extensively in the quantitation of nitrate and nitrite using fluorescence spectroscopy.2,3 Upon entry into the cell, DAN-1 EE is transformed into the less cell permeable DAN-1 by cellular esterases thus preventing loss of signal due to diffusion of the molecule from the cell. Intracellular formation of NO can be monitored using excitation and emission wavelengths of 360-380 nm and 420-450 nm, respectively.1

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Kojima, H., Sakurai, K., Kikuchi, K., et al. Development of a fluorescent indicator for the bioimaging of nitric oxide. Biol. Pharm. Bull. 20, 1229-1232 (1997).

    2. Miles, A.M., Chen, Y., Owens, M.W., et al. Fluorometric determination of nitric oxide. Methods 7(1), 40-47 (1995).

    3. Misko, T.P., Schilling, R.J., Salvemini, D., et al. A fluorometric assay for the measurement of nitrite in biological samples. Anal. Biochem. 214, 11-16 (1993).