A fluorogenic peptide substrate
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Gly-Gly-AMC (trifluoroacetate salt)

Item No. 85140

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
glycyl-N-(4-methyl-2-oxo-2H-1-benzopyran-7-yl)-glycinamide
Molecular Formula
C14H15N3O4 • XCF3COOH
Formula Weight
289.3
Purity
≥98%
Emission
440-460 nm
Excitation
340-360 nm
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMF: 1 mg/mlDMSO: 20 mg/mlEthanol: 1 mg/mlPBS (pH 7.2): 0.8 mg/ml
λmax
327 nm
SMILES
NCC(N([H])CC(N([H])C1=CC=C(C(C)=CC(O2)=O)C2=C1)=O)=O.O=C(O)C(F)(F)F
InChi Code
InChI=1S/C14H15N3O4.C2HF3O2/c1-8-4-14(20)21-11-5-9(2-3-10(8)11)17-13(19)7-16-12(18)6-15;3-2(4,5)1(6)7/h2-5H,6-7,15H2,1H3,(H,16,18)(H,17,19);(H,6,7)
InChi Key
FNZVLKOGEFWXPZ-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    1 mg

    $36

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    5 mg

    $153

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    10 mg

    $268

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    25 mg

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    Product Description

    Gly-Gly-AMC is a fluorogenic peptide substrate. It has been used to assess the activity of bacterial proteases from P. aeruginosa and S. aureus.1 Activity can be quantified by fluorescent detection of free AMC (also known as 7-amino-4-methylcoumarin), which is excited at 340-360 nm and emits at 440-460 nm.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Wildeboer, D., Jeganathan, F., Price, R.G., et al. Characterization of bacterial proteases with a panel of fluorescent peptide substrates. Anal. Biochem. 384(2), 321-328 (2009).