A fluorescent probe used for the detection of NO
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Product Categories

Product Type

Biochemicals
Labeling & Detection
Fluorescent Probes
Reactive Probes

Research Area

Application

Fluorescence

DAF-2

Item No. 85160

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
2-(3,6-dihydroxy-4,5-diamino-9H-xanthen-9-yl)-benzoic acid
CAS Number
205391-01-1
Synonyms
  • 4,5-Diaminofluorescein
Molecular Formula
C20H14N2O5
Formula Weight
362.3
Purity
≥98%
Emission
538 nm
Excitation
485 nm
Formulation (Request formulation change)
A 500 µg/ml solution in DMSO
SMILES
OC1=CC2=C(C=C1)C3(C(C=C(N)C(N)=C4)=C4C(O3)=O)C5=CC=C(O)C=C5O2
InChi Code
InChI=1S/C20H14N2O5/c21-15-7-11-14(8-16(15)22)20(27-19(11)25)12-3-1-9(23)5-17(12)26-18-6-10(24)2-4-13(18)20/h1-8,23-24H,21-22H2
InChi Key
LTYUPYUWXRTNFQ-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    100 µg

    $68

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    250 µg

    $154

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    500 µg

    $291

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    1 mg

    $515

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    Product Description

    DAF-2 is a sensitive fluorescent indicator commonly used for the detection of nitric oxide (NO).1,2 It reacts with NO in the presence of oxygen to yield the highly fluorescent triazolofluorescein (DAF-2T). Fluorescence is monitored using excitation and emission wavelengths of 485 and 538 nM, respectively.2 At neutral pH the detection limit for NO is 2-5 nM.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Kojima, H., Sakurai, K., Kikuchi, K., et al. Development of a fluorescent indicator for nitric oxide based on the fluorescein chromophore. Chem. Pharm. Bull. (Tokyo) 46(2), 373-375 (1998).

    2. Nakatsubo, N., Kojima, H., Kikuchi, K., et al. Direct evidence of nitric oxide production from bovine aortic endothelial cells using new fluorescence indicators: Diaminofluoresceins. FEBS Lett. 427(2), 263-266 (1998).