A reagent and hapten
Technical Support & Resources

Information provided in the product description is from published literature. Due to the nature of scientific experimentation, your results (e.g., selectivity and effective concentrations) or specific application for this product may differ. If you have questions about how this product fits your application, please contact our technical support staff.

Visit our FAQ

Contact Us

Toll Free Phone (USA and Canada Only): (888) 526-5351
Direct Phone: (734) 975-3888

Request Technical Support

Technical Support Request

To streamline the process attach the appropriate questionnaire to your inquiry.

Download IHC QuestionnaireDownload WB Questionnaire

View Our Privacy Statement for details on how we use and protect your data. In addition, this site is protected by hCaptcha and its Privacy Policy and Terms of Service apply.

2,4,6-Trinitrobenzenesulphonic Acid

Item No. 9004605

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
2,4,6-trinitro-benzenesulfonic acid
CAS Number
2508-19-2
Synonyms
  • NSC 127994
  • Picrylsulfonic Acid
  • TNBS
Molecular Formula
C6H3N3O9S
Formula Weight
293.2
Formulation (Request formulation change)
A 100 mg/ml solution 10% (w/v) in water
SMILES
O=S(C1=C([N+]([O-])=O)C=C(C=C1[N+]([O-])=O)[N+]([O-])=O)(O)=O
InChi Code
InChI=1S/C6H3N3O9S/c10-7(11)3-1-4(8(12)13)6(19(16,17)18)5(2-3)9(14)15/h1-2H,(H,16,17,18)
InChi Key
NHJVRSWLHSJWIN-UHFFFAOYSA-N
Shipping & Storage Information
Storage
4°C
Shipping
Wet ice in continental US; may vary elsewhere
Recommended Products

Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

Download most recent Certificate of Analysis for this product

    50 mg

    $299

    Add

    100 mg

    $568

    Add
    Product Description

    2,4,6-Trinitrobenzenesulfonic acid (TNBS) is a reagent and hapten.1,2 It reacts with free amino groups in peptides and proteins to produce a compound that absorbs at 335 nm and has been used in this way for the quantification of free amino groups during protein hydrolysis.1 As a hapten, TNBS binds to high-molecular weight molecules, such as proteins, inducing them to become immunogenic.2 In this way, it has commonly been used to induce colonic inflammation in laboratory animals as a model of inflammatory bowel disease (IBD), including Crohn's disease and ulcerative colitis.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Akhtar, M., and Ding, R. Covalently cross-linked proteins & polysaccharides: Formation, characterisation and potential applications. Curr. Opin. Colloid Interface Sci. 53:101450, (2017).

    2. Antoniou, E., Margonis, G.A., Angelou, A., et al. The TNBS-induced colitis animal model: An overview. Ann. Med. Surg. (Lond) 11, 9-15 (2016).