These resources are provided to serve the analysis needs of researchers using our Lipoxygenase Inhibitor Screening Assay Kit.
If you have questions on the use of our tools, or feel something is missing, please contact Technical Services.
Currently, Cayman Chemical uses two Microsoft Excel workbooks for analyzing hydrogen peroxide assays: LISTriple and LISDouble. The first is formatted for assays performed in triplicate, and the second is formatted for assays performed in duplicate.
Your downloading these files implies acceptance of the license agreement listed below and present in each workbook.
Trouble downloading? Right-click the link and select "Save Target As..."
These instructions are repeated on the first sheet of each workbook.
LISTriple Worksheet
LISDouble Worksheet
| Term | Definition |
|---|---|
| BLANK | Serves as a negative control |
| Positive Ctrl | Positive control provided in the kit |
| 100% Init. Activity | Initial activity of the 15-lipoxygenase standard provided in the kit |
| Smpl# (1:#) | Samples and their associated dilution factors |
| Field | Explanation |
|---|---|
| LAYOUT | Contains sample names and associated dilution factors |
| RAW DATA | Absorbance value (between 490-500 nm) of each well after two 5 minute incubations |
| ACTIVITY | For each absorbance value, the activity of the well is calculated using the method on page 15 of the kit booklet |
| RESULTS | This table is compiled from the preceding sections. It includes the sample name, assay dilution and percent inhibition of the compound. The inhibition is calculated using the method on page 16 in the kit booklet |
| FILENAME | The filename given to the Excel workbook |
| VERSION | The version of the workbook |
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