TP Receptor (human) Polyclonal Antibody

Thromboxane A₂ (TXA₂) is a potent vasoconstrictor and activator of platelet aggregation. TXA₂ elicits its effects via a 7-transmembrane domain G protein-coupled receptor, the TP receptor.¹ The TP receptor is highly expressed in platelets and is relatively less abundant in tissues such as lung, kidney, brain, spleen, thymus, monocytes, uterus, and placenta.^2,3,4,5 The apparent molecular weight for TP receptors has been reported from 37 kDa to 70 kDa, depending on different degrees of glycosylation.^5,6,7 However Cayman’s antibody consistently detects the TP receptor at 55 and 64 kDa in platelet, kidney, and Cos-7 cell samples. Cayman Chemical’s TP receptor polyclonal antibody can be used for western blot and immunocytochemical analysis for both the TPA and TPB splice variants of the receptor on samples of human, murine, rat, and COS-7 (African green monkey) origin.

WARNING This product is not for human or veterinary use.

1. Hirata, M., Hayashi, Y., Ushikubi, F., et al. Cloning and expression of cDNA for a human thromboxane A₂ receptor. Nature 349(6310), 617-620 (1991).

2. Namba, T., Sugimoto, Y., Hirata, M., et al. Mouse thromboxane A₂ receptor: cDNA cloning, expression and Northern blot analysis. Biochem. Biophys. Res. Commun. 184(3), 1197-1203 (1992).

3. Båtshake, B., Nilsson, C., and Sundelin, J. Structure and expression of the murine thromboxane A₂ receptor gene. Biochem. Biophys. Res. Commun. 256(2), 391-397 (1999).

4. Mais, D.E., True, T.A., and Martinelli, M.J. Characterization by photoaffinity labelling of the human platelet thromboxane A₂/prostaglandin H₂ receptor: Evidence for N-linked glycosylation. Eur. J. Pharmacol. 227(3), 267-274 (1992).

5. Takahashi, N., Takeuchi, K., Abe, T., et al. Immunolocalization of rat thromboxane receptor in the kidney. Endocrinology 137(11), 5170-5173 (1996).

6. Wang, G.-R., Zhu, Y., Halushka, P.V., et al. Mechanism of platelet inhibition by nitric oxide: In vivo phosphorylation of thromboxane receptor by cyclic GMP-dependent protein kinase. Proc. Natl. Acad. Sci. USA 95(9), 4888-4893 (1998).

7. Habib, A., Fitzgerald, G.A., and Maclouf, J. Phosphorylation of the thromboxane receptor α, the predominant isoform expressed in human platelets. The Journal of Biological Chemisty 274(5), 2645-2651 (1999).

Zhang, Y., Mi, M., Xie, Y.-H., et al. Downregulation of thromboxane A₂ receptor occurs mainly via nuclear factor-kappaB signaling pathway in rat renal artery. Adv. Pharmacol. Sci. 2017, 6507048 (2017).

Li, H., Liu, K., Boardman, L.A., et al. Circulating prostaglandin biosynthesis in colorectal cancer and potential clinical significance. EBioMedicine 2(2), 165-171 (2015).