For immunochemical detection of 11β-HSD2
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11β-Hydroxysteroid Dehydrogenase (Type 2) Polyclonal Antibody

Item No. 10004549

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • 11β-HSD2
  • Corticosteroid 11β-Dehydrogenase Isozyme 2
Immunogen
Synthetic peptide from the N-terminal region of human 11β-HSD2
MW
44 kDa
Formulation (Request formulation change)
500 µl of peptide affinity-purified polyclonal antibody
Storage Buffer
PBS, pH 7.2, with 50% glycerol and 0.02% sodium azide
Host
Rabbit
Applications
IHC, WB
Cross Reactivity
(+) 11β-Hydroxysteroid Dehydrogenase (Type 2)(+) 11β-Hydroxysteroid Dehydrogenase (Type 1)
Species Reactivity
(+) Human(+) Mouse(+) Rat
UniProt Accession №
P80365
Origin
Animal/Rabbit
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    1 ea (500 µl)

    $347

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    Product Description

    11β-Hydroxysteroid Dehydrogenase (Type 2) (11β-HSD2) catalyzes the conversion of cortisol (hydrocortisone; Item No. 20739) to the inactive glucocorticoid cortisone (Item No. 30763), thereby protecting the mineralcorticoid receptor from glucocorticoid excess.1 It is primarily expressed in the kidneys, but has also been detected in the colon, salivary glands, and fetal tissues, including the placenta, and is localized to the endoplasmic reticulum.1,2 Knockout of HSD11B2, the gene encoding 11β-HSD2, reduces fetal and placental growth as well as capillary development in mice.3 Loss-of-function mutations in HSD11B2 result in apparent mineralcorticoid excess (AME), an inborn error of metabolism characterized by hypertension, hypokalemia, and reduced plasma renin activity.1 Cayman's 11β-Hydroxysteroid Dehydrogenase (Type 2) Polyclonal Antibody can be used for immunohistochemistry (IHC) and Western blot (WB) applications. The antibody recognizes 11β-HSD2 at 44 kDa from mouse samples.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. White, P.C., Mune, T., and Agarwal, A.K. 11β-hydroxysteroid dehydrogenase and the syndrome of apparent mineralocorticoid excess. Endocr. Rev. 18(1), 135-156 (1997).

    2. Náray-Fejes-Tóth, A., and Fejes-Tóth, G. Subcellular localization of the type 2 11β-hydroxysteroid dehydrogenase. A green fluorescent protein study. The Journal of Biological Chemisty 271(26), 15436-15442 (1996).

    3. Wyrwoll, C.S., Seckl, J.R., and Holmes, M.C. Altered placental function of 11β-hydroxysteroid dehydrogenase 2 knockout mice. Endocrinology 150(3), 1287-1293 (2009).