For immunochemical detection of cytokeratins 4, 5, 6, 8, 10, 13, and 18
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Cytokeratin Monoclonal Antibody (Clone C-11)

Item No. 10004600

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • CKs
  • pan-Cytokeratin
  • Keratin
Clone Designation
C-11
Formulation (Request formulation change)
100 μg of ammonium sulfate-purified monoclonal antibody
Storage Buffer
PBS, pH 7.2
Host
Mouse
Isotype
IgG1
Applications
FC, IF, IHC, WB
Cross Reactivity
(+) Human cytokeratin 4(+) Human cytokeratin 5(+) Human cytokeratin 6(+) Human cytokeratin 8(+) Human cytokeratin 10(+) Human cytokeratin 13(+) Human cytokeratin 18
Species Reactivity
(+) Human cytokeratin
Origin
Animal/Mouse
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    1 ea (100 µg)

    $347

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    Product Description

    Cytokeratins (CKs) are intermediate filaments proteins and members of the cytokeratin protein family.1,2,3 The cytokeratin family is composed of at least 20 unique gene products, which fall into two categories: type I acidic cytokeratins, CK9-CK20, and type II neutral-basic cytokeratins, CK1-CK8.2 Each cytokeratin is composed of a central rod containing four α-helical domains, which are important for self-assembly, and non-helical head and tail domains at the N- and C-termini, respectively.2,3 Cytokeratins are major structural proteins expressed in epithelial cells and localized to the cytoplasm where they form heterozygous type I-type II pairs.1,4 They primarily function to protect epithelial cells from mechanical and non-mechanical stresses that induce cell rupture and death.5 Cytokeratins have additional isoform- and tissue-specific functions, including the modulation of protein synthesis and cell size during epithelial growth and roles in skin pigmentation. Cayman’s Cytokeratin Monoclonal Antibody (Clone C-11) can be used for flow cytometry (FC), immunofluorescence (IF), immunohistochemistry (IHC), and Western blot (WB) applications. This antibody recognizes cytokeratins 4, 5, 6, 8, 10, 13, and 18 from human samples.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Weng, Y.-R., Cui, Y., and Fang, J.-Y. Biological functions of cytokeratin 18 in cancer. Mol. Cancer Res. 10(4), 485-493 (2012).

    2. Gu, L.-H., and Coulombe, P.A. Keratin function in skin epithelia: A broadening palette with surprising shades. Curr. Opin. Cell Biol. 19(1), 13-23 (2007).

    3. Eldirany, S.A., Lomakin, I.B., Ho, M., et al. Recent insight into intermediate filament structure. Curr. Opin. Cell Biol. 68, 132-143 (2021).

    4. Ditzel, H.J., Strik, M.C.M., Larsen, M.K., et al. Cancer-associated cleavage of cytokeratin 8/18 heterotypic complexes exposes a neoepitope in human adenocarcinomas. The Journal of Biological Chemisty 277(24), 21712-21722 (2002).

    5. Tanny, J.C., and Moazed, D. Coupling of histone deacetylation to NAD breakdown by the yeast silencing protein Sir2: Evidence for acetyl transfer from substrate to an NAD breakdown product. Proc. Natl. Acad. Sci. USA 98(2), 415-420 (2001).