A calcium ionophore
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Alternative(s)
11932Ionomycin (calcium salt)
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Ionomycin

Item No. 10004974

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
(4R,6S,8S,10Z,12R,14R,16E,18R,19R,20S,21S)-11,19,21-trihydroxy-4,6,8,12,14,18,20-heptamethyl-22-[(2S,2'R,5S,5'S)-octahydro-5'-[(1R)-1-hydroxyethyl]-2,5'-dimethyl[2,2'-bifuran]-5-yl]-9-oxo-10,16-docosadienoic acid
CAS Number
56092-81-0
Molecular Formula
C41H72O9
Formula Weight
709.0
Purity
≥95%
Formulation (Request formulation change)
A 10 mg/ml solution in ethanol
Solubility(Learn about Variance in Solubility)
DMF: 2.5 mg/mlDMSO: 1.4 mg/mlEthanol: 20 mg/mlEthanol:PBS (pH 7.2) (1:10): 0.1 mg/ml
λmax
277 nm
SMILES
C[C@@H](O)[C@](O1)(C)CC[C@]1([H])[C@]2(C)CCC(C[C@H](O)[C@H](C)[C@H](O)[C@H](C)/C=C/C[C@@H](C)C[C@@H](C)/C(O)=C/C([C@@H](C)C[C@@H](C)C[C@H](C)CCC(O)=O)=O)O2
InChi Code
InChI=1S/C41H72O9/c1-25(21-29(5)34(43)24-35(44)30(6)22-27(3)20-26(2)14-15-38(46)47)12-11-13-28(4)39(48)31(7)36(45)23-33-16-18-41(10,49-33)37-17-19-40(9,50-37)32(8)42/h11,13,24-33,36-37,39,42-43,45,48H,12,14-23H2,1-10H3,(H,46,47)/b13-11+,34-24-/t25-,2
InChi Key
PGHMRUGBZOYCAA-KCZOVTJBSA-N
Origin
Bacterium/Streptomyces conglobatus
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

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    1 mg

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    NET Formation Screening & Analysis Services
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    Product Description

    Ionomycin is a calcium ionophore originally isolated from S. conglobatus.1 It induces intracellular calcium mobilization in X. laevis oocytes when used at a concentration of 5 µM. Ionomycin (5 µM) induces the formation of neutrophil extracellular traps (NETs) in isolated human neutrophils.2 Ionomycin in combination with phorbol 12-myristate 13-acetate (PMA; Item No. 10008014) has commonly been used to induce the proliferation and activation of T cells, as well as to stimulate cytokine production in macrophages, in vitro.3,4

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Liu, W.C., Slusarchyk, D.S., Astle, G., et al. Ionomycin, a new polyether antibiotic. J. Antibiot. (Tokyo) 31(9), 815-819 (1978).

    2. Locke, M., Francis, R.J., Tsaousi, E., et al. Fibrinogen protects neutrophils from the cytotoxic effects of histones and delays neutrophil extracellular trap formation induced by ionomycin. Sci. Rep. 10(1), 11694 (2020).

    3. Lehnert, C., Weiswange, M., Jeremias, I., et al. TRAIL-receptor costimulation inhibits proximal TCR signaling and suppresses human T cell activation and proliferation. J. Immunol. 193(8), 4021-4031 (2014).

    4. Foey, A.D., and Brennan, F.M. Conventional protein kinase C and atypical protein kinase Cζ differentially regulate macrophage production of tumour necrosis factor-ɑ and interleukin-10. Immunology 112(1), 44-53 (2004).

    Product Citations

    Ning, X., Wang, Y., Jing, M., et al. Apoptotic caspases suppress type I interferon production via the cleavage of cGAS, MAVS, and IRF3. Mol. Cell 74(1), 19-31 (2019).

    Soni, H., and Adebiyi, A. TRPC6 channel activation promotes neonatal glomerular mesangial cell apoptosis via calcineurin/NFAT and FasL/Fas signaling pathways. Sci. Rep. 6, 29041 (2016).

    Bosnakovski, D., Choi, S.H., Strasser, J.M., et al. High-throughput screening identifies inhibitors of DUX4-induced myoblast toxicity. Skelet. Muscle 4(4), (2014).