A calcium ionophore
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Ionomycin (calcium salt)

Item No. 11932

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
11,19R,21S-trihydroxy-4R,6S,8S,12R,14R,18R,20S-heptamethyl-22-[octahydro-5'S-[1R-hydroxyethyl]-2S,5'-dimethyl[2,2'R-bifuran]-5S-yl]-9-oxo-10Z,16E-docosadienoic acid, calcium salt
CAS Number
56092-82-1
Molecular Formula
C41H70O9 • Ca
Formula Weight
747.1
Purity
≥98%
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMF: 1.2 mg/mlDMSO: 1.6 mg/mlEthanol: 20 mg/mlEthanol:PBS(pH 7.2) (1:1): 0.5 mg/ml
λmax
296 nm
SMILES
C[C@@H](O)[C@](O1)(C)CC[C@]1([H])[C@]2(C)CC[C@](C[C@H](O)[C@H](C)[C@H](O)[C@H](C)/C=C/C[C@@H](C)C[C@@H](C)/C([O-])=C/C([C@@H](C)C[C@@H](C)C[C@H](C)CCC([O-])=O)=O)([H])O2.[Ca+2]
InChi Code
InChI=1S/C41H72O9.Ca/c1-25(21-29(5)34(43)24-35(44)30(6)22-27(3)20-26(2)14-15-38(46)47)12-11-13-28(4)39(48)31(7)36(45)23-33-16-18-41(10,49-33)37-17-19-40(9,50-37)32(8)42;/h11,13,24-33,36-37,39,42-43,45,48H,12,14-23H2,1-10H3,(H,46,47);/q;+2/p-2/b13-11+
InChi Key
WKRWUYKLUMMAKG-WYGBAUISSA-L
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    NET Formation Screening & Analysis Services
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    Product Description

    Ionomycin is a calcium ionophore originally isolated from S. conglobatus.1 It induces intracellular calcium mobilization in X. laevis oocytes when used at a concentration of 5 µM. Ionomycin (5 µM) induces the formation of neutrophil extracellular traps (NETs) in isolated human neutrophils.2 Ionomycin in combination with phorbol 12-myristate 13-acetate (PMA; Item No. 10008014) has commonly been used to induce the proliferation and activation of T cells, as well as to stimulate cytokine production in macrophages, in vitro.3,4

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Liu, W.C., Slusarchyk, D.S., Astle, G., et al. Ionomycin, a new polyether antibiotic. J. Antibiot. (Tokyo) 31(9), 815-819 (1978).

    2. Locke, M., Francis, R.J., Tsaousi, E., et al. Fibrinogen protects neutrophils from the cytotoxic effects of histones and delays neutrophil extracellular trap formation induced by ionomycin. Sci. Rep. 10(1), 11694 (2020).

    3. Lehnert, C., Weiswange, M., Jeremias, I., et al. TRAIL-receptor costimulation inhibits proximal TCR signaling and suppresses human T cell activation and proliferation. J. Immunol. 193(8), 4021-4031 (2014).

    4. Foey, A.D., and Brennan, F.M. Conventional protein kinase C and atypical protein kinase Cζ differentially regulate macrophage production of tumour necrosis factor-ɑ and interleukin-10. Immunology 112(1), 44-53 (2004).