An immunometric ELISA for analyzing Vtg in whole body homogenates and plasma samples
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  • An immunometric ELISA for analyzing Vtg in whole body homogenates and plasma samples
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Vitellogenin (zebrafish) ELISA Kit

Item No. 10004995

Technical Information
Synonyms
  • Vitellogenin (zebrafish) EIA
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4°C
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Wet ice in continental US; may vary elsewhere
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    Product Description

    Detection of the yolk protein Vtg in plasma from juvenile or male fish is a simple and sensitive biomarker for endocrine disrupting chemicals (EDCs) with estrogenic effects in fish. Quantification of Vtg has become an accepted screening test for the estrogenic effects of EDCs in fish. The Vtg (zebrafish) ELISA kit is a double-antibody immunometric (sandwich) ELISA for analyzing Vtg in whole body homogenates and plasma samples. Each kit contains zebrafish Vtg, pre-coated plate(s), detecting antibody, secondary antibody-HRP conjugate, BSA, OPD-peroxidase substrate, PBS-Tween tablets, PBS tablets, and complete instructions. A five-plate kit contains 2 vials of Vtg and 3 OPD sets, sufficient to run two separate assays (for example 2 plates + 3 plates).

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Citations

    Sullivan, K.M., Park, C.G., Ito, J.D., et alMatrix softness-mediated 3D zebrafish hepatocyte modulates response to endocrine disrupting chemicals. Environ. Sci. Technol. 54(21), 13797-13806 (2020).

    Zhu, Y., Hua, R., Zhou, Y., et alChronic exposure to mono-(2-ethylhexyl)-phthalate causes endocrine disruption and reproductive dysfunction in zebrafish. Environ. Toxicol. Chem. 35(8), 2117-2124 (2016).

    Fang, Q., Shi, Q., Guo, Y., et alEnhanced bioconcentration of bisphenol A in the presence of nano-TiO2 can lead to adverse reproductive outcomes in zebrafish. Environ. Sci. Technol. 50(2), 1005-1013 (2016).