Protein Carbonyl Colorimetric Assay Kit

Item № 10005020
SIZE PRICE QUANTITY SUBTOTAL
CART TOTAL $0.00
96 wells $275.00 0.00

Pricing updated 2018-02-25. Prices are subject to change without notice.

Description
Features
  • Measure protein carbonyl content in plasma, serum, urine, tissue homogenates, and cell lysates
  • Assay 24 samples in duplicate
  • Plate-based colorimetric measurement (360-385 nm)

Cayman’s Protein Carbonyl Colorimetric Assay Kit is a convenient colorimetric assay for the measurement of oxidized proteins. Protein samples are derivatized by making use of the reaction between 2,4-dinitrophenylhydrazine and protein carbonyls. Formation of a Schiff base produces the corresponding hydrazone which can be analyzed spectrophotometrically at 360-385 nm. This assay can be used to measure oxidized protein in plasma, serum, cell lysates, and tissue homogenates.

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

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Technical Information

Warning - this product is not for human or veterinary use.

Shipping & Storage
Storage
4°C
Shipping
Wet ice in continental US; may vary elsewhere
Stability
≥ 1 year
Downloads & Resources
Product Downloads

Download Kit Booklet

Download Safety Data Sheet (SDS)

Download free InChI Key generation software

Additional Information

View the Cayman Structure Database for chemical structure definitions for many Cayman products

Get Batch-Specific Data and Documents by Batch Number

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data pack, and GC-MS data.

Technical Support
FAQ

For the Protein Carbonyl Assay Kit are there recommendations for use of samples dissolved in buffer that is 30 mM Tris-HCl, 7 M Urea, 2 M Thiourea, 4% CHAPS, Protease inhibitor cocktail, pH 8.5?

The buffer you are using is designed to solubilize proteins. It is possible that the protein in your samples will have a difficult time precipitating in the assay. The carbonyl groups in urea and CHAPS should not react with the DNPH in the assay, but it is unknown whether or not these components will interfere (as other substances that contain no carbonyl groups can still interfere, such as Triton X-100). The assay may suit your needs but we cannot guarantee this.

For the Protein Carbonyl Assay Kit You indicate the following equation; Protein Carbonyl(nmol/ml)=[(CA)/(*0.011uM^-1)](500ul/200ul) Does an actual calculation simply divide CA by 0.011? What do you mean "(500 µl/200 µl)" ?

Dividing CA by 0.011µM^-1 will give the concentration of protein carbonyl for the 500µl guanidine hydrochloride solution. Performing the 500µl/200µl will give the concentration of protein carbonyl in the original 200µl sample. If a customer only used a 100µl sample, for example, the equation would be adjusted to 500µl/100µl. This term in the equation accounts for sample dilution.

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You may be eligible to receive a free sample of Protein Carbonyl Colorimetric Assay Kit under the Cayman Challenge program.

Cayman Chemical

1180 East Ellsworth Road

Ann Arbor, Michigan 48108 USA

Toll Free: (800) 364-9897

(USA and Canada Only)

Fax: (734) 971-3640