Pricing updated 2018-09-18. Prices are subject to change without notice.
Cayman’s Protein Carbonyl Colorimetric Assay Kit is a convenient colorimetric assay for the measurement of oxidized proteins. Protein samples are derivatized by making use of the reaction between 2,4-
Warning - this product is not for human or veterinary use.
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For the Protein Carbonyl Assay Kit are there recommendations for use of samples dissolved in buffer that is 30 mM Tris-HCl, 7 M Urea, 2 M Thiourea, 4% CHAPS, Protease inhibitor cocktail, pH 8.5?
The buffer you are using is designed to solubilize proteins. It is possible that the protein in your samples will have a difficult time precipitating in the assay. The carbonyl groups in urea and CHAPS should not react with the DNPH in the assay, but it is unknown whether or not these components will interfere (as other substances that contain no carbonyl groups can still interfere, such as Triton X-100). The assay may suit your needs but we cannot guarantee this.
For the Protein Carbonyl Assay Kit You indicate the following equation; Protein Carbonyl(nmol/ml)=[(CA)/(*0.011uM^-1)](500ul/200ul) Does an actual calculation simply divide CA by 0.011? What do you mean "(500 µl/200 µl)" ?
Dividing CA by 0.011µM^-1 will give the concentration of protein carbonyl for the 500µl guanidine hydrochloride solution. Performing the 500µl/200µl will give the concentration of protein carbonyl in the original 200µl sample. If a customer only used a 100µl sample, for example, the equation would be adjusted to 500µl/100µl. This term in the equation accounts for sample dilution.