LPA₁ Polyclonal Antibody

LPA₁ (also known as EDG-2) is one of four known LPA receptors (LPA_1-4).¹ LPA_1-3 are part a family of G protein-coupled receptors that share identity to the sphingosine-1-phosphate receptors (S1P_1-5).² LPA₁ couples with three types of G proteins, G_i/o, G_q, and G_12/13 to induce a range of cellular responses including activation of phospholipase C, multiple kinases, the serum response element and cell proliferation.^3,4 LPA₁ mRNA is detected a wide range of tissues and cells including brain, heart, small intestine, spleen, testis, kidney, HeLa cells, and A549 cells.^5,6 Mouse and human LPA₁ have 364 amino acids with an estimated molecular weight of 41 kDa.⁴ Cayman’s LPA₁ Polyclonal Antibody detects the protein from several species from 43-50 kDa, suggesting heterogeneous LPA₁ post-translational modifications in distinct cells.

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1. Noguchi, K., Ishii, S., and Shimizu, T. Identification of p2y9/GPR23 as a novel G protein-coupled receptor for lysophosphatidic acid, structurally distant from the Edg family. The Journal of Biological Chemisty 278(28), 25600-25606 (2003).

2. Chun, J., Goetzl, E.J., Hla, T., et al. International union of pharmacology. XXXIV. Lysophospholipid receptor nomenclature. Pharmacol. Rev. 54(2), 265-269 (2002).

3. Fukushima, N., and Chun, J. The LPA receptors. Prostaglandins Other Lipid Mediat. 64, 21-32 (2001).

4. Ishii, I., Fukushima, N., Ye, X., et al. Lysophospholipid receptors: Signaling and biology. Annu. Rev. Biochem. 73, 321-354 (2004).

5. Contos, J.J.A., and Chun, J. The mouse lpA3/Edg7 lysophosphatidic acid receptor gene: genomic structure, chromosomal localization, and expression pattern. Gene 267, 243-253 (2001).

6. An, S., Bleu, T., Hallmark, O.G., et al. Characterization of a novel subtype of human G protein-coupled receptor for lysophosphatidic acid. The Journal of Biological Chemisty 273(14), 7906-7910 (1998).

Sinderewicz, E., Grycmacher, K., Boruszewska, D., et al. Expression of factors involved in apoptosis and cell survival is correlated with enzymes synthesizing lysophosphatidic acid and its receptors in granulosa cells originating from different types of bovine ovarian follicles. Reprod. Biol. Endocrinol. 15(1), 72 (2017).

Suckau, O., Gross, I., Schrötter, S., et al. LPA₁, LPA₂, LPA₄, and LPA₆ receptor expression during mouse brain development. Dev. Dyn. 248(5), 375-395 (2019).