LPA₁ Blocking Peptide

To be used in conjunction with Cayman’s LPA₁ polyclonal antibody (Catalog No. 10005280) to block protein-antibody complex formation during immunochemical analysis of LPA₁. · LPA₁ (also known as EDG-2) is one of four known LPA receptors (LPA_1-4).¹ LPA_1-3 are part a family of G protein-coupled receptors that share identity to the sphingosine-1-phosphate receptors (S1P_1-5).² LPA₁ couples with three types of G proteins, G_i/o, G_q, and G_12/13 to induce a range of cellular responses including activation of phospholipase C, multiple kinases, the serum response element, and cell proliferation.^3,4 LPA₁ mRNA is detected in a wide range of tissues and cells including brain, heart, small intestine, spleen, testis, kidney, HeLa cells, and A549 cells.^5,6 Murine and human LPA₁ have 364 amino acids with an estimated molecular weight of 41 kDa.⁴ Cayman’s LPA₁ polyclonal antibody detects the protein from several species at 43-50 kDa, suggesting heterogeneous LPA₁ post-translational modifications in distinct cells.

WARNING This product is not for human or veterinary use.

1. Noguchi, K., Ishii, S., and Shimizu, T. Identification of p2y9/GPR23 as a novel G protein-coupled receptor for lysophosphatidic acid, structurally distant from the Edg family. The Journal of Biological Chemisty 278(28), 25600-25606 (2003).

2. Chun, J., Goetzl, E.J., Hla, T., et al. International union of pharmacology. XXXIV. Lysophospholipid receptor nomenclature. Pharmacol. Rev. 54(2), 265-269 (2002).

3. Fukushima, N., and Chun, J. The LPA receptors. Prostaglandins Other Lipid Mediat. 64, 21-32 (2001).

4. Ishii, I., Fukushima, N., Ye, X., et al. Lysophospholipid receptors: Signaling and biology. Annu. Rev. Biochem. 73, 321-354 (2004).

5. Contos, J.J.A., and Chun, J. The mouse lpA3/Edg7 lysophosphatidic acid receptor gene: genomic structure, chromosomal localization, and expression pattern. Gene 267, 243-253 (2001).

6. An, S., Bleu, T., Hallmark, O.G., et al. Characterization of a novel subtype of human G protein-coupled receptor for lysophosphatidic acid. The Journal of Biological Chemisty 273(14), 7906-7910 (1998).

Klomsiri, C., Rogers, L.C., Soito, L., et al. Endosomal H₂O₂ production leads to localized cysteine sulfenic acid formation on proteins during lysophosphatidic acid-mediated cell signaling. Free Rad. Biol. Med. 71, 49-60 (2014).