Specifically measures the octanoylated active form of ghrelin
Features
  • Measure acetylated ghrelin in mouse or rat blood, plasma, or serum samples
  • Assay 24 samples in triplicate or 36 samples in duplicate
  • Incubation: 3 hours
  • Sampling tubes with PHMB available to store, preserve, and protect samples from protein degradation (see Item No. 10949)
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Acylated Ghrelin (mouse, rat) Express EIA Kit

Item No. 10006307

Technical Information
Synonyms
  • Ghrelin (rat acylated) ELISA
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    Product Description

    Ghrelin, an endogenous ligand for the growth hormone secretagogue receptor, is synthesized principally in the stomach. It stimulates food intake and transduces signals to hypothalamic regulatory nuclei that control energy homeostasis. The peptide consists of 28 amino acids, with an octanoylation of the serine-3 residue, which is necessary for biological activity. Ghrelin is present in the peripheral circulation in two forms: acylated and non-acylated. This EIA kit specifically measures the acylated form of ghrelin. Each kit contains ghrelin (acylated) AChE-Fab' conjugate, rat acylated ghrelin standard, rat acylated ghrelin quality control, Ellman’s reagent, EIA buffer, wash buffer, Tween 20, plates pre-coated with anti-ghrelin mouse monoclonal antibody, and complete instructions. [Bertin Catalog No. A05117]

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Citations

    Li, V.L., He, Y., Contrepois, K., et alAn exercise-inducible metabolite that suppresses feeding and obesity. Nature 606, 785-790 (2022).

    McFarlane, M.R., Brown, M.S., Goldstein, J.L., et alInduced ablation of ghrelin cells in adult mice does not decrease food intake, body weight, or response to high-fat diet. Cell Metab. 20(1), 54-60 (2014).