A fast, fluorescence-based method for evaluating PCAF HAT inhibitors
Features
  • Screen for inhibitors of PCAF
  • Assay 46 inhibitor samples in duplicate
  • Plate-based fluorometric measurement (ex 360-390 nm, em 450-470 nm)
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HAT Inhibitor Screening Assay Kit

Item No. 10006515

Kit Booklet (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Histone Acetyltransferase
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    96 wells

    $383

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    Product Description

    Cayman’s Histone Acetyltransferase (HAT) Inhibitor Screening Assay Kit provides a fast, fluorescence-based method for evaluating PCAF HAT inhibitors. The procedure requires only three easy steps, all performed in the same microwell plate. In the first step of the protocol, HAT is incubated with acetyl-CoA and the histone H3 peptide. During this time, HAT catalyzes the enzymatic transfer of acetyl groups from acetyl-CoA to the H3 peptide producing an acetylated peptide and CoASH. Following addition of isopropanol to stop the enzymatic reaction, CPM is added to the wells of the plate. CPM reacts with the free thiol groups present on CoASH forming a highly fluorescent product that is detected using excitation and emission wavelengths of 360-390 and 450-470 nm, respectively.

    Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Citations

    Ding, Y., Ko, M.H., Pehar, M., et al. Biochemical inhibition of the acetyltransferases ATase1 and ATase2 reduces b-secretase (BACE1) levels and Ab generation. The Journal of Biological Chemisty 287(11), 8424-8433 (2012).