Host: E. coli • AA: 1-199 (full length) • Tag: N-terminal His • MW: 24.3 kDa
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Prostaglandin D Synthase (hematopoietic-type; human, recombinant)

Item No. 10006593

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • H-PGDS
  • hH-PGDS
  • Hematopoietic-PGDS
  • PGD Synthase (hematopoietic-type; human recombinant)
Purity
≥95% estimated by SDS-PAGE
Source
Recombinant human N-terminal His-tagged H-PGDS expressed in E. coli
Amino Acids
1-199 (full length)
MW
24.3 kDa
Formulation (Request formulation change)
50 mM sodium phosphate, pH 7.2, containing 20% glycerol, 100 mM sodium chloride, 1 mM DTT, and 0.5 mM EDTA
UniProt Accession №
O60760
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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    100 µg

    $423

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    Product Description

    Prostaglandin D synthase (PGDS) is a glutathione-dependent enzyme and member of the sigma class of glutathione-S-transferases (GSTs) that catalyzes the conversion of PGH2 (Item No. 17020) to PGD2 (Item No. 12010), an eicosanoid that has numerous biological functions, including vasorelaxation, recruitment of inflammatory cells, and inhibition of platelet aggregation.1,2,3 There are two types of PGDS: lipocalin PGDS (L-PGDS; Item No. 10006788) and hematopoietic PGDS (H-PGDS; Item No. 10006593).3 H-PGDS is found in peripheral tissues and immune cells, including Th2 cells, antigen-presenting cells, mast cells, megakaryocytes, and eosinophils, where it is localized to the cytosol.2 H-PGDS activity is increased by a variety of stimuli, including LPS, anti-IgE antibodies, phorbol 12-myristate 13-acetate (TPA; Item No. 10008014), ionomycin (Item No. 10004974), and inflammatory cytokines such as IL-13, IL-3, or IL-4.3 siRNA silencing of Hpgds decreases LPS-induced production of PGD2 in mouse bone marrow-derived macrophages (BMDMs).4 Transgenic overexpression of HPGDS in mice increases croton oil-induced ear swelling and PGD2 production, and genome-wide deletion of Hpgds exacerbates hypotension and vascular permeability in a mouse model of anaphylaxis.5,6 H-PGDS protein levels are increased in the nasal mucosa of patients with allergic rhinitis, and HPGDS SNPs have been found in individuals with asthma.3,1 Cayman's Prostaglandin D Synthase (hematopoietic-type; human, recombinant) can be used for enzyme activity assays.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Kanaoka, Y., and Urade, Y. Hematopoietic prostaglandin D synthase. Prostaglandins Leukot. Essent. Fatty Acids 69(2-3), 163-167 (2003).

    2. Thurairatnam, S. Hematopoietic prostaglandin D synthase inhibitors. Prog. Med. Chem. 51, 97-133 (2012).

    3. Rittchen, S., and Heinemann, A. Therapeutic potential of hematopoietic prostaglandin D2 synthase in allergic inflammation. Cells 8(6), 619 (2019).

    4. Zhao, G., Yu, R., Deng, J., et al. Pivotal role of reactive oxygen species in differential regulation of lipopolysaccharide-induced prostaglandins production in macrophages. Mol. Pharmacol. 83(1), 167-178 (2013).

    5. Sarashina, H., Tsubosaka, Y., Omori, K., et al. Opposing immunomodulatory roles of prostaglandin D2 during the progression of skin inflammation. J. Immunol. 192(1), 459-465 (2014).

    6. Nakamura, T., Fujiwara, Y., Yamada, R., et al. Mast cell-derived prostaglandin D2 attenuates anaphylactic reactions in mice. J. Allergy Clin. Immunol. 140(2), 630-632 (2017).

    Product Citations

    Johnsson, A.K., Choi, J.H., Rönnberg, E., et al. Selective inhibition of prostaglandin D2 biosynthesis in human mast cells to overcome need for multiple receptor antagonists: Biochemical consequences. Clin. Exp. Allergy 51(4), 594-603 (2021).