Autotaxin Blocking Peptide

To be used in conjunction with Cayman’s autotaxin polyclonal antibody (Item No. 10005375) to block protein-antibody complex formation during immunochemical analysis of lyso-PLD. Lysophosphatidic acid (LPA) is an extracellular signaling lipid that evokes multiple biological functions including induction of platelet aggregation, smooth muscle contraction, and stimulation of cell proliferation and chemotaxis.¹ Lyso-PLD was first discovered as the enzyme responsible for generating LPA from lysophosphatidylcholine (LPC).² It was later revealed to be identical to an autocrine motility factor, autotaxin (ATX), which plays a role in tumor progression and metastasis.^3,4 Lyso-PLD/ATX mRNA is widely expressed with highest levels found in brain, ovary, lung, intestine, and testis.^5,6 Cayman’s autotaxin polyclonal antibody can be used for WB and ICC analysis for lyso-PLD on samples of human, murine, and rat origin. Other applications have not yet been tested.

WARNING This product is not for human or veterinary use.

1. Ishii, I., Fukushima, N., Ye, X., et al. Lysophospholipid receptors: Signaling and biology. Annu. Rev. Biochem. 73, 321-354 (2004).

2. Tokumura, A., Majima, E., Kariya, Y., et al. Identification of human lysophospholipase D, a lysophosphatidic acid-producing enzyme, as autotaxin, a multifunctional phosphodiesterase. The Journal of Biological Chemisty 277(42), 39436-39442 (2002).

3. Umezu-Goto, M., Kishi, Y., Tairia, A., et al. Autotaxin has lysophospholipase D activity leading to tumor cell growth and motility by lysophosphatidic acid production. J. Cell Biol. 158, 227-233 (2005).

4. Nam, S.W., Clair, T., Campo, C.K., et al. Autotaxin (ATX), a potent tumor mitogen, augments invasive and metastatic potential of ras-transformed cells. Oncogene 19, 241-247 (2000).

5. Sun, Y.X., Tsubio, K., Okamoto, Y., et al. Biosynthesis of anandamide and N-palmitoylethanolamide by sequential actions of phospholipase A₂ and lysophosphase D. J. Biochem. 380(Pt 3), 749-755 (2004).

6. van Leeuwen, F.N., Giepmanst, B.N.G., van Meeteren, L.A., et al. Lysophosphatidic acid: Mitogen and motility factor. Biochem. Soc. Trans. 31, 1209-1212 (2003).

Iyer, P., Lalane, R., III, Morris, C., et al. Autotaxin-lysophosphatidic acid axis is a novel molecular target for lowering intraocular pressure. PLoS One 7(8), e42627 (2012).