For immunochemical detection of SREBP-2
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Alternative(s)
27231SREBP-2 Monoclonal Antibody (Clone 1B7)
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SREBP-2 Polyclonal Antibody

Item No. 10007663

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • SREBF2
  • Sterol Regulatory Element-binding Protein-2
  • Sterol Regulatory Element-binding Transcription Factor 2
Immunogen
Synthetic peptide from an internal region of human SREBP-2
Formulation (Request formulation change)
500 μl of peptide-affinity purified polyclonal antibody
Storage Buffer
PBS, pH 7.2, with 50% glycerol and 0.02% sodium azide
Host
Rabbit
Applications
IF, IHC, and WB
Species Reactivity
(+) Human(+) Mouse(+) Rat
UniProt Accession №
Q12772
Origin
Animal/Rabbit
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

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    1 ea (500 μl)

    $402

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    Product Description

    SREBPs, including SREBP-1a, SREBP-1c, and SREBP-2, constitute a family of basic helix-loop-helix (bHLH) transcription factors that play a critical role in lipid homeostasis by regulating genes involved in cholesterol and fatty acid metabolism.1 SREBP-2 regulates cholesterol synthesis by activating the transcription of genes for HMG-CoA reductase and other enzymes of the cholesterol synthetic pathway.2 SREBP-2 is ubiquitously detected in various tissues.3 Upon cholesterol depletion, the protein is cleaved to its active forms (about 50-68 kDa) and translocated into the nucleus to stimulate transcription of genes involved in the uptake and synthesis of cholesterol.4 Cayman’s SREBP-2 polyclonal antibody detects both precursor and active forms of the protein in tissues and cells such as liver, brown fat, testis, HepG2 cells, and human fibroblast. The apparent molecular weight on SDS-PAGE may be higher than the calculated molecular weight (about 126 kDa) due to glycosylation of the protein.5

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Brown, M.S., and Goldstein, J.L. The SREBP pathway: Regulation of cholesterol metabolism by proteolysis of a membrane-bound transcription factor. Cell 89(3), 331-340 (1997).

    2. Sakai, J., Nohturfft, A., Goldstein, J.L., et al. Cleavage of sterol regulatory element-binding proteins (SREBPs) at site-1 requires interaction with SREBP cleavage-activating protein. Evidence from in vivo competition studies. The Journal of Biological Chemisty 273(10), 5785-5793 (1998).

    3. Iizuka, K., Bruick, R.K., Liang, G., et al. Deficiency of carbohydrate response element-binding protein (ChREBP) reduces lipogenesis as well as glycolysis. Proc. Natl. Acad. Sci. USA 101(19), 7281-7286 (2004).

    4. Smith, L.H., Petrie, M.S., Morrow, J.D., et al. The sterol response element binding protein regulates cyclooxygenase-2 gene expression in endothelial cells. J. Lipid Res. 46, 862-871 (2005).

    5. Hua, X., Sakai, J., Ho, Y.K., et al. Hairpin orientation of sterol regulatory element-binding protein-2 in cell membranes as determined by protease protection. The Journal of Biological Chemisty 270(49), 29422-29427 (1995).

    Product Citations

    Mahmud, I., Tian, G., Wang, J., et al. DAXX drives de novo lipogenesis and contributes to tumorigenesis. Nat. Commun. 14(1), 1927 (2023).