SREBP-2 Monoclonal Antibody (Clone 1B7)

Sterol regulatory element-binding protein-2 (SREBP-2) is a membrane-bound transcription factor encoded by the SREBF2 gene in humans and is involved in the regulation of cholesterol biosynthesis and uptake.^1,2,3 Similar to SREBP-1a and SREBP-1c, SREBP-2 is comprised of an N-terminal acidic domain, a basic-helix-loop-helix leucine zipper (bHLH-Zip) sequence, two transmembrane segments, and a regulatory C-terminal domain.¹ In the absence of sterols, SREBP-2 undergoes two sequential proteolytic cleavage events resulting in release of the bHLH-Zip-containing N terminus from the endoplasmic reticulum membrane, followed by translocation to the nucleus.^1,2 Nuclear SREBP-2 (nSREBP-2) binds to sterol response elements (SREs) in DNA and regulates expression of genes encoding HMG-CoA synthase, HMG-CoA reductase, farnesyl diphosphate synthase, and squalene synthase, among others.² Srebp2 is ubiquitously expressed in mouse tissues and knockout of Srebp2 is embryonic lethal in mice.^4,2 Hepatic expression of SREBF2 is seven- and three-fold higher in patients with non-alcoholic steatohepatitis (NASH) and steatosis, respectively, than that of healthy individuals.⁵ Cayman's SREBP-2 Monoclonal Antibody (Clone 1B7) can be used for immunocytochemistry, immunohistochemistry, and western blot applications.

WARNING This product is not for human or veterinary use.

1. Brown, M.S., and Goldstein, J.L. The SREBP pathway: Regulation of cholesterol metabolism by proteolysis of a membrane-bound transcription factor. Cell 89(3), 331-340 (1997).

2. Horton, J.D., Goldstein, J.L., and Brown, M.S. SREBPs: Activators of the complete program of cholesterol and fatty acid synthesis in the liver. J. Clin. Invest. 109(9), 1125-1131 (2002).

3. Eberlé, D., Hegarty, B., Bossard, P., et al. SREBP transcription factors: Master regulators of lipid homeostasis. Biochimie 86(11), 839-848 (2004).

4. Iizuka, K., Bruick, R.K., Liang, G., et al. Deficiency of carbohydrate response element-binding protein (ChREBP) reduces lipogenesis as well as glycolysis. Proc. Natl. Acad. Sci. USA 101(19), 7281-7286 (2004).

5. Caballero, F., Fernández, A., De Lacy, A.M., et al. Enhanced free cholesterol, SREBP-2 and StAR expression in human NASH. J. Hepatol. 50(4), 789-796 (2009).