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Serum Retinol Binding Protein 4 (human recombinant)

Item No. 10007818

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • RBP4
  • sRBP4
Purity
≥95%
Source
Recombinant His-tagged protein purified from E. coli
MW
~21 kDa
Formulation (Request formulation change)
50 mM sodium phosphate, pH 8.2, with 100 mM sodium chloride and 20% glycerol
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    100 µg

    $589

    Add
    Cayman Chemical
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    Product Description

    Human serum retinol binding protein 4 (sRBP4) binds to one equivalent of vitamin A and is one of the major retinol carriers found in the blood of mammals.1,2 Human sRBP4 is a monomeric 21 kDa β-sheet-rich protein that contains three disulfide bonds and belongs to the lipocalin protein family.3 In plasma, sRBP4 typically forms a 1:1 complex with the 55 kDa tetrameric protein transthyretin (TTR) which prevents RBP from being removed from the plasma by glomerular filtration.4 Recent studies have shown that sRBP4 is an adipocyte-derived “signal” that may contribute to the pathogenesis of type 2 diabetes.5,6 Elevation of sRBP4 causes systemic insulin resistance, whereas reduced serum concentrations of sRBP4 improves insulin action.5,7,8 Cayman’s human recombinant sRBP4 contains a C-terminal hexahistidine tag. The purified protein was characterized for its retinol binding activity.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Noy, N. Retinoid-binding proteins: Mediators of retinoid action. Biochem. J. 348(Pt. 3), 481-495 (2000).

    2. Xie, Y., Lashuel, H.A., Miroy, G.J., et al. Recombinant human retinol-binding protein refolding, native disulfide formation, and characterization. Protein Expr. Purif. 14(1), 31-37 (1998).

    3. Cowan, S.W., Newcomer, M.E., and Jones, T.A. Crystallographic refinement of human serum retinol binding protein at 2Å resolution. Proteins 8(1), 44-61 (1990).

    4. Sivaprasadarao, A., and Findlay, J.B.C. Expression of functional human retinol-binding protein in Escherichia coli using a secretion vector. Biochem. J. 296(Pt. 1), 209-215 (1993).

    5. Yang, Q., Graham, T.E., Mody, N., et al. Serum retinol binding protein 4 contributes to insulin resistance in obesity and type 2 diabetes. Nature 436(7049), 356-362 (2005).

    6. Muoio, D.M., and Newgard, C.B. Metabolism: A is for adipokine. Nature 436(7049), 337-338 (2005).

    7. Graham, T.E., Yang, Q., Blüher, M., et al. Retinol-binding protein 4 and insulin resistance in lean, obese, and diabetic subjects. N. Engl. J. Med. 354(24), 2552-2563 (2006).

    8. Polonsky, K.S. Retinol-binding protein 4, insulin resistance, and type 2 diabetes. N. Engl. J. Med. 354(24), 2596-2598 (2006).