A 96-well assay for measurement of NF-κB (p65)
Features
  • A sensitive, non-radioactive method of detecting NF-κB (p65) from whole cell lysates
  • 96-well plate format replaces EMSAs
  • Capture the transcription factor using a specific dsDNA sequence bound to the plate
  • Detect the dsDNA-bound transcription factor with specific antibodies in an ELISA format
  • Nuclear Extraction Kit (Item No. 10009277) available to aid in the isolation of nuclear and cytoplasmic fractions from cell lysates or tissue homogenates
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NF-κB (p65) Transcription Factor Assay Kit

Item No. 10007889

Kit Booklet (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Origin
Animal/Bovine, Animal/Goat, Animal/Rabbit
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    96 wells

    $600

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    Product Description

    Cayman’s NF-κB (p65) Transcription Factor Assay is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts and whole cell lysates. A 96-well enzyme-linked immunosorbent assay (ELISA) replaces the cumbersome radioactive electrophoretic mobility shift assay (EMSA). A specific double stranded DNA (dsDNA) sequence containing the NF-κB response element is immobilized to the wells of a 96-well plate. NF-κB contained in a nuclear extract, binds specifically to the NF-κB response element. NF-κB (p65) is detected by addition of specific primary antibody directed against NF-κB (p65). A secondary antibody conjugated to HRP is added to provide a sensitive colorimetric readout at 450 nm. Cayman’s NF-κB (p65) Transcription Factor Assay detects human NF-κB (p65). It will not cross-react with NF-κB (p50).

    Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Citations

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    Li, H., Wang, Y., Zhang, H., et al. Yohimbine enhances protection of berberine against LPS-induced mouse lethality through multiple mechanisms. PLoS One 7(12), (2012).

    Zaki, H., Vogel, P., Malireddi, R.K.S., et al. The NOD-like receptor NLRP12 attenuates colon inflammation and tumorigenesis. Cancer Cell 20(5), 649-660 (2011).