Analysis of mitochondrial membrane potential using fluorescence microscopy or a fluorescent plate reader
Features
  • Measure mitochondrial membrane potential as an indicator of cell health
  • Utilizes JC-1, a fluorescent, lipophilic, cationic dye
  • Red fluorescence indicates healthy mitochondria and green fluorescence indicates mitochondria in poor health
  • Optimized for fluorescence microscopy or fluorescent plate readers. An assay optimized for flow cytometry is available under Item No. 701560.
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JC-1 Mitochondrial Membrane Potential Assay Kit

Item No. 10009172

Kit Booklet (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    100 tests

    $308

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    Product Description

    Mitochondrial membrane potential, (ΔψM), is an important parameter of mitochondrial function used as an indicator of cell health. JC-1 is a lipophilic, cationic dye that can selectively enter into mitochondria and reversibly change color from green to red as the membrane potential increases. In healthy cells with high mitochondrial (ΔψM), JC-1 spontaneously forms complexes known as J-aggregates with intense red fluorescence. On the other hand, in apoptotic or unhealthy cells with low (ΔψM), JC-1 remains in the monomeric form, which shows only green fluorescence. Cayman’s JC-1 Mitochondrial Membrane Potential Assay Kit provides all the necessary reagents, as well as complete instructions, for analysis of mitochondrial integrity in whole cells. The assay is applicable to fluorescence microscopy and fluorescent plate reader. For flow cytometry, Cayman’s JC-1 Mitochondrial Membrane Potential Flow Cytometry Assay Kit (Item No. 701560) is recommended.

    Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Citations

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    Ayers, S., Graf, T.N., Adcock, A.F., et al. Resorcylic acid lactones with cytotoxic and NF-κB inhibitory activities and their structure-activity relationships. J. Nat. Prod. 74(5), 1126-1131 (2011).