Flow cytometric analysis of mitochondrial membrane potential using a ratiometric dye
Features
  • Measure mitochondrial membrane potential as an indicator of cell health
  • Utilizes JC-1, a fluorescent, lipophilic, and cationic dye
  • Includes FCCP as a compensation control
  • Optimized specifically for flow cytometry. An assay optimized for fluorescence microscopy or fluorescent plate readers is available under Item No. 10009172.
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JC-1 Mitochondrial Membrane Potential Flow Cytometry Assay Kit

Item No. 701560

Kit Booklet (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    500 tests

    $381

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    Product Description

    Cayman’s JC-1 Mitochondrial Membrane Potential Flow Cytometry Assay Kit can be used to study mitochondrial behavior in a variety of conditions, including apoptosis. Changes in ΔψM, reflected by aggregation level of JC-1, can be determined as a ratio of red to green mean fluorescence intensities using flow cytometry. Flow cytometry is an ideal way to assess the JC-1 aggregation at the single-cell level, and provides the added benefit of potential to multiplex other readouts from the same cells.3 This kit additionally contains FCCP for treatment of cells as a compensation control. The reagents in this kit are sufficient for staining up to 500 samples for JC-1.

    Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Citations

    Agrawal, K., Bouchal, J., Das, V., et al. Laboratory techniques in cellular and molecular medicine. (2021).