For immunochemical detection of MEK1/2
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MEK1/2 (Phospho-Ser218,222) Polyclonal Antibody

Item No. 10009178

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • MAP Kinase Kinase 1/2
  • MAPKK1/2
  • Mitogen-activated Protein Kinase Kinase 1/2
Immunogen
phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser218,222 of human MEK1/2
Formulation (Request formulation change)
Affinity-purified IgG
Host
Rabbit
Applications
WB
Species Reactivity
(+) NIH 3T3 cells
Origin
Animal/Rabbit
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    $464

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    Product Description

    MAP kinase kinase 1 (MEK1) is an integral component of the MAP kinase cascade that regulates cell growth and differentiation.1,2 This pathway also plays a key role in synaptic plasticity in the brain.3 Activated MEK1 acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on MAP kinase.4,5,6

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Ahn, N.G. The MAP kinase cascade. Discovery of a new signal transduction pathway. Mol. Cell Biochem. 127-128, 201-209 (1993).

    2. Chong, H., Vikis, H.G., and Guan, K.L. Mechanisms of regulating the Raf kinase family. Cell. Signal. 15(5), 463-469 (2003).

    3. Adams, J.P., and Sweatt, J.D. Molecular psychology: Roles for the ERK MAP kinase cascade in memory. Annu. Rev. Pharmacol. Toxicol. 42, 135-163 (2002).

    4. Mansour, S.J., Resing, K.A., Candi, J.M., et al. Mitogen-activated protein (MAP) kinase phosphorylation of MAP kinase kinase: Determination of phosphorylation sites by mass spectrometry and site-directed mutagenesis. J. Biochem. 116(2), 304-314 (1994).

    5. Kyriakis, J.M., Brautigan, D.L., Ingebritsen, T.S., et al. pp54 Microtubule-associated protein-2 kinase requires both tyrosine and serine/threonine phosphorylation for activity. The Journal of Biological Chemisty 266(16), 10043-10046 (1991).

    6. Seger, R., Ahn, N.G., Boulton, T.G., et al. Microtubule-associated protein 2 kinases, ERK1 and ERK2, undergo autophosphorylation on both tyrosine and threonine residues: Implications for their mechanism of activation. Proc. Natl. Acad. Sci. USA 88(14), 6142-6146 (1991).