For immunochemical detection of MEK1
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MEK1 (Phospho-Thr386) Polyclonal Antibody

Item No. 10009517

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • MAP Kinase Kinase 1
  • MAPKK1
  • Mitogen-activated Protein Kinase Kinase 1
Immunogen
phosphopeptide corresponding to amino acid residues surrounding the Phospho-Thr386 of human MEK1
Formulation (Request formulation change)
Affinity-purified IgG
Host
Rabbit
Applications
WB
Species Reactivity
(+) Rat MEK 1(+) Bovine MEK1 (expected)(+) Canine MEK1 (expected)(+) Chicken MEK1 (expected)(+) Mouse MEK1 (expected)(+) Non-human primates MEK1 (expected)(+) Xenopus MEK1 (expected)
Origin
Animal/Rabbit
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

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    $464

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    Product Description

    MAP kinase kinase 1 (MEK1) is an integral component of the MAP kinase cascade that regulates cell growth and differentiation 1,2. This pathway also plays a key role in synaptic plasticity in the brain 3. Activated MEK1 acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on MAP kinase 4,5,6. Conversely, there also appears to be a feedback phosphorylation of MEK1 by MAP kinase. The sites on MEK1 that are phosphorylated by MAP kinase are Thr292 and Thr386 7.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Ahn, N.G. The MAP kinase cascade. Discovery of a new signal transduction pathway. Mol. Cell Biochem. 127-128, 201-209 (1993).

    2. Chong, H., Vikis, H.G., and Guan, K.L. Mechanisms of regulating the Raf kinase family. Cell. Signal. 15(5), 463-469 (2003).

    3. Adams, J.P., and Sweatt, J.D. Molecular psychology: Roles for the ERK MAP kinase cascade in memory. Annu. Rev. Pharmacol. Toxicol. 42, 135-163 (2002).

    4. Kyriakis, J.M., Brautigan, D.L., Ingebritsen, T.S., et al. pp54 Microtubule-associated protein-2 kinase requires both tyrosine and serine/threonine phosphorylation for activity. The Journal of Biological Chemisty 266(16), 10043-10046 (1991).

    5. Seger, R., Ahn, N.G., Boulton, T.G., et al. Microtubule-associated protein 2 kinases, ERK1 and ERK2, undergo autophosphorylation on both tyrosine and threonine residues: Implications for their mechanism of activation. Proc. Natl. Acad. Sci. USA 88(14), 6142-6146 (1991).

    6. Crews, C.M., Alessandrini, A., and Erikson, R.L. The primary structure of MEK a protein kinase that phosphorylates the ERK gene product (extracellular signal-regulated kinases). Science 258(5081), 478-480 (1992).

    7. Mansour, S.J., Resing, K.A., Candi, J.M., et al. Mitogen-activated protein (MAP) kinase phosphorylation of MAP kinase kinase: Determination of phosphorylation sites by mass spectrometry and site-directed mutagenesis. J. Biochem. 116(2), 304-314 (1994).