Simple, reproducible, and sensitive tool for measurement of glycerol in plasma and serum
Features
  • Measure glycerol in plasma and serum
  • Assay 40 samples in duplicate
  • Plate-based colorimetric measurement (530-550 nm)
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Glycerol Colorimetric Assay Kit

Item No. 10010755

Technical Information
Limit of Detection
0.2 mg/L
Lower Limit of Quantification
2.0 mg/L
Origin
Plant/Armoracia rusticana
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

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    Cayman Chemical
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    Product Description

    Glycerol is the backbone of triglycerides and an important intermediate in energy metabolism being involved in both oxidative and synthetic processes. The measurement of circulating levels of glycerol and free fatty acids are considered to reflect lipolysis and are therefore useful parameters to evaluate in various conditions. The measurements of glycerol is also useful for correction of glycerol interference when measuring triglycerides in reference materials and in serum from patients with elevated glycerol concentrations. Cayman’s Glycerol Assay provides a simple, reproducible, and sensitive tool for measurement of glycerol in plasma and serum. The assay employs a coupled enzymatic reaction system that yields a brilliant purple product with an absorbance maximum at 540 nm.

    Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Citations

    Iwamoto, K., Kamo, S., Takada, Y., et alSoyasapogenols reduce cellular triglyceride levels in 3T3-L1 mouse adipocyte cells by accelerating triglyceride lipolysis. Biochem. Biophys. Rep. 16, 44-49 (2018).

    Alharthi, A., Zhou, Z., Lopreiato, V., et alBody condition score prior to parturition is associated with plasma and adipose tissue biomarkers of lipid metabolism and inflammation in Holstein cows. J. Anim. Sci. Biotechnol. 9, (2018).

    Furno, D.L., Graziano, A.C.E., Avola, R., et alA Citrus bergamia extract decreases adipogenesis and increases lipolysis by modulating PPAR levels in mesenchymal stem cells from human adipose tissue. PPAR Res. 2016, 4563815 (2016).

    Ghoshal, S., Zhu, Q., Astelan, A., et alTNP [N2-(m-trifluorobenzyl), N6-(p-nitrobenzyl)purine] ameliorates diet induced obesity and insulin resistance via inhibition of the IP6K1 pathway. Mol. Metab. 5(10), 903-907 (2016).

    Xiao, C., Goldgof, M., Gavrilova, O., et alAnti-obesity and metabolic efficacy of the b3-adrenergic agonist, CL316243, in mice at thermoneutrality compared to 22°C. Obesity 23(7), 1450-1459 (2015).