SREBP-1 Transcription Factor Assay Kit

Three known isoforms of SREBP (sterol regulatory element binding protein) transcription factors have been characterized: SREBP-1a, SREBP-1c, and SREBP-2. SREBP-1c acts primarily to activate genes required for fatty acid synthesis, such as acetyl-CoA carboxylase, fatty acid synthase, and long chain fatty acid elongase. In addition, SREBP-1c may also contribute to the regulation of glucose uptake and synthesis through induction of glucokinase. SREBP-1c has important clinical implications in the treatment of many diseases including obesity, diabetes mellitus, insulin resistance, and non-alcoholic fatty liver disease (NAFLD). Cayman’s SREBP-1 Transcription Factor Assay is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity. SREBP-1 contained in nuclear extracts and whole cell lysates binds to a dsDNA SREBP response element immobilized to the wells of a 96-well plate. SREBP-1 is detected by addition of a specific primary antibody directed against SREBP-1. A secondary antibody conjugated to HRP is used to provide a sensitive colorimetric readout at 450 nm.

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

WARNING This product is not for human or veterinary use.

Jang, J., Jung, Y., Seo, S.J., et al. Berberine activates AMPK to suppress proteolytic processing, nuclear translocation and target DNA binding of SREBP-1c in 3T3-L1 adipocytes. Mol. Med. Rep. 15(6), 4139-4147 (2017).

Valenzuela, R., Echeverria, F., Ortiz, M., et al. Hydroxytyrosol prevents reduction in liver activity of D-5 and D-6 desaturases, oxidative stress, and depletion in long chain polyunsaturated fatty acid content in different tissues of high-fat diet fed mice. Lipids Health Dis. 16:64, (2017).