Active • Host: Insect cells • AA: 2-555 (full length) • Tag: N-terminal His • MW: 64 kDa
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Soluble Epoxide Hydrolase (human, recombinant)

Item No. 10011669

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Cytosolic Epoxide Hydrolase (CEH)
  • EPHX2
  • Epoxide Hydrolase 2
  • sEH
Purity
≥95% estimated by SDS-PAGE
Source
Active recombinant human N-terminal His-tagged sEH expressed in insect cells
Amino Acids
2-555 (full length)
MW
64 kDa
Formulation (Request formulation change)
TBS, pH 7.4, with 20% glycerol
Host
Insect cells
UniProt Accession №
P34913
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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    50 µg

    $439

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    Product Description

    Soluble epoxide hydrolase (sEH) is a member of the α/β-hydrolase fold enzyme family that catalyzes the hydrolysis of bioactive fatty acid epoxides to inactive vicinal diols.1 It is a homodimer in which each subunit is composed of two domains, a C-terminal epoxide hydrolase domain and an N-terminal phosphatase domain.2,3 sEH is localized to the cytoplasm or to peroxisomes in a tissue-specific manner and is found in various tissues, including skin, lung, uterus, kidney, brain, and myocardium.4,5 sEH is also expressed in the vasculature and inhibition of sEH attenuates pathogenic vascular remodeling and hypertension via preservation of cardioprotective epoxyeicosatrienoic acids (EETs) in rat models of atherosclerosis and hypertension, respectively.6 Inhibition of sEH also has a protective role in various diseases, including inflammatory bowel disease, osteoarthritis, seizure, stroke, and Alzheimer’s disease, as well as in various chronic pain states.1,7 Cayman’s Soluble Epoxide Hydrolase (human, recombinant) protein can be used for enzyme activity assays.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Harris, T.R., and Hammock, B.D. Soluble epoxide hydrolase: Gene structure, expression and deletion. Gene 526(2), 61-74 (2013).

    2. Cronin, A., Mowbray, S., Dürk, H., et al. The N-terminal domain of mammalian soluble epoxide hydrolase is a phosphatase. Proc. Natl. Acad. Sci. USA 100(4), 1552-1557 (2002).

    3. Chiamvimonvat, N., Ho, C.M., Tsai, H.J., et al. The soluble epoxide hydrolase as a pharmaceutical target for hypertension. J. Cardiovasc. Pharmacol. 50(3), 225-237 (2007).

    4. Enayetallah, A.E., French, R.A., Barber, M., et al. Cell-specific subcellular localization of soluble epoxide hydrolase in human tissues. J. Histochem. Cytochem. 54(3), 329-335 (2006).

    5. Domingues, M.F., Callai-Silva, N., Piovesan, A.R., et al. Soluble epoxide hydrolase and brain cholesterol metabolism. Front. Mol. Neurosci. 12, 325 (2020).

    6. Simpkins, A.N., Rudic, R.D., Roy, S., et al. Soluble epoxide hydrolase inhibition modulates vascular remodeling. Am. J. Physiol. Heart Circ. Physiol. 298(3), H795-H806 (2009).

    7. Wagner, K.M., McReynolds, C.B., Schmidt, W.K., et al. Soluble epoxide hydrolase as a therapeutic target for pain, inflammatory and neurodegenerative diseases. Pharmacol. Ther. 180, 62-76 (2017).

    Product Citations

    Adebesin, A.M., Wesser, T., Vijaykumar, J., et al. Development of robust 17(R),18(S)-epoxyeicosatetraenoic acid (17,18-EEQ) analogues as potential clinical antiarrhythmic agents. J. Med. Chem. 62(22), 10124-10143 (2019).