An inhibitor of PLA2 from red blood cells
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EA4

Item No. 10111

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
7-chloro-6-[4-(diethylamino)phenyl]-5,8-quinolinedione
CAS Number
389614-94-2
Molecular Formula
C19H17ClN2O2
Formula Weight
340.8
Purity
≥95%
Formulation (Request formulation change)
A crystalline solid
λmax
202, 242, 280, 600 nm
SMILES
CCN(CC)c1ccc(cc1)C1=C(Cl)C(=O)c2ncccc2C1=O
InChi Code
InChI=1S/C19H17ClN2O2/c1-3-22(4-2)13-9-7-12(8-10-13)15-16(20)19(24)17-14(18(15)23)6-5-11-21-17/h5-11H,3-4H2,1-2H3
InChi Key
XAZVKSMDPVMMPP-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    5 mg

    $94

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    10 mg

    $150

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    50 mg

    $609

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    100 mg

    $1,029

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    Product Description

    rPLA2 is a calcium-dependent cytosolic phospholipase A2 (cPLA2) initially isolated and characterized from bovine and human red blood cells (RBCs).1 It has a molecular mass of 42 kDa and biochemical properties similar to cPLA2 Type IV. EA4 is a quinone derivative that inhibits rPLA2 with a Ki of 130 µM. It inhibits the ionophore-induced arachidonic acid release from human and bovine red blood cells (RBCs), indicating that rPLA2 is responsible for the Ca2+-dependent release of arachidonic acid from mammalian RBCs.1 EA4 causes significant time- and concentration-dependent induction of cytochrome P450 1A1 (CYP1A1) mRNA and protein in murine hepatoma Hepa-1c1c7 cells.2 EA4 also modulates CYP1A1 and CYP1B1 expression in other cell lines such as MCF-7, Hep-G2, and HL-60.2

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Shin, H.S., Chin, M.R., Kim, J.S., et al. Purification and characterization of a cytosolic, 42-kDa and Ca2+-dependent phospholipase A2 from bovine red blood cells. The Journal of Biological Chemisty 277(23), 21086-21094 (2002).

    2. Chun, Y.J., Lee, B.Y., Yang, S.A., et al. Induction of cytochrome P450 1A1 gene expression by a vitamin K3 analog in mouse hepatoma Hepa-1c1c7 cells. Mol. Cells 12(2), 190-196 (2001).