A colorimetric FAAH substrate for enzyme activity measurement
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Arachidonoyl p-Nitroaniline

Item No. 10168

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
N-(4-nitrophenyl)-5Z,8Z,11Z,14Z-eicosatetraenamide
CAS Number
119520-58-0
Synonyms
  • ApNA
Molecular Formula
C26H36N2O3
Formula Weight
424.6
Purity
≥98%
Formulation (Request formulation change)
A 10 mg/ml solution in methyl acetate
Solubility(Learn about Variance in Solubility)
DMF: 50 mg/mlDMSO: 50 mg/mlDMSO:PBS (pH 7.2) (1:4): 1 mg/mlEthanol: 50 mg/ml
λmax
204, 316 nm
SMILES
CCCCC/C=C\C/C=C\C/C=C\C/C=C\CCCC(=O)Nc1ccc(cc1)[N](=O)O
InChi Code
InChI=1S/C26H36N2O3/c1-2-3-4-5-6-7-8-9-10-11-12-13-14-15-16-17-18-19-26(29)27-24-20-22-25(23-21-24)28(30)31/h6-7,9-10,12-13,15-16,20-23H,2-5,8,11,14,17-19H2,1H3,(H,27,29)/b7-6-,10-9-,13-12-,16-15-
InChi Key
PBMVYDNBOIVHBO-DOFZRALJSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

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    1 mg

    $18

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    5 mg

    $70

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    10 mg

    $120

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    50 mg

    $516

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    Product Description

    Arachidonoyl p-nitroaniline (ApNA) is one of several nitroaniline fatty acid amides which can be used to measure fatty acid amide hydrolase (FAAH) activity.1 FAAH is a relatively unselective enzyme in that it accepts a variety of amide head groups other than the ethanolamine of its nominal endogenous substrate anandamide (AEA; Item No. 90050). It also will hydrolyze fatty acid amides with fewer carbons and fewer double bonds than arachidonate. (See also Decanoyl p-Nitroaniline - Catalog No. 90349). Exposure of ApNA to FAAH activity results in the release of the yellow colorimetric dye p-nitroaniline (ε = 13,500 at 382 nm). This offers the potential for fast and convenient measurements of FAAH activity using a 96-well plate spectrophotometer.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Patricelli, M.P., and Cravatt, B.F. Characterization and manipulation of the acyl chain selectivity of fatty acid amide hydrolase. Biochemistry 40(20), 6107-6115 (2001).