PPARδ Polyclonal Antibody

PPARδ is one of three peroxisome proliferator-activated receptor (PPAR) subtypes that possess a domain structure common to other members of the nuclear receptor gene family. It was first cloned from Xenopus laevis and named PPARβ.¹ PPARδ is ubiquitously expressed but is particularly abundant in tissues such as liver, intestine, kidney, abdominal adipose, and skeletal muscle, all of which are involved in lipid metabolism.² PPARδ is a mediator of diverse physiological functions including lipid and cholesterol homeostasis, embryo implantation, and cancer development.^3,4,5,6 Most recently, attention has been focused on the role of PPARδ in obesity.⁷ Cayman’s PPARδ polyclonal antibody can be used for Western blot and immunohistochemistry to study the expression and functions of this protein. The antibody recognizes PPARδ at 50 kDa from human samples. An additional smaller size of PPARδ (~ 40 kDa)⁸ is also detected in certain mouse tissues.

WARNING This product is not for human or veterinary use.

1. Dreyer, C., Krey, G., Keller, H., et al. Control of the peroxisomal β-oxidation pathway by a novel family of nuclear hormone receptors. Cell 68, 879-887 (1992).

2. Willson, T.M., Brown, P.J., Sternbach, D.D., et al. The PPARs: From orphan receptors to drug discovery. J. Med. Chem. 43(4), 527-550 (2000).

3. Amri, E.Z., Bonino, F., Ailhaud, G., et al. Cloning of a protein that mediates transcriptional effects of fatty acids in preadipocytes. Homology to peroxisome proliferator-activated receptors. The Journal of Biological Chemisty 270, 2367-2371 (1995).

4. Berger, J., Leibowitz, M.D., Doebber, T.W., et al. Novel peroxisome proliferator-activated receptor (PPAR) γ and PPARδ ligands produce distinct biological effects. The Journal of Biological Chemisty 574(10), 6718-6725 (1999).

5. Lim, H., and Dey, S.K. PPARδ functions as a prostacyclin receptor in blastocyst implantation. Trends Endocrinol. Metab. 11(4), 137-142 (2000).

6. He, T.-C., Chan, T.A., Vogelstein, B., et al. PPARδ is an APC-regulated target of nonsteroidal anti-inflammatory drugs. Cell 99(3), 335-345 (1999).

7. Wang, Y.-X., Lee, C.-H., Tiep, S., et al. Peroxisome-proliferator-activated receptor δ activates fat metabolism to prevent obesity. Cell 113(2), 159-170 (2003).

8. Mammalian Gene Collection Program, T. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Proc. Natl. Acad. Sci. USA 99(26), 16899-16903 (2002).

Reddy, A.T., Lakshmi, S.P., Banno, A., et al. Identification and Molecular Characterization of PPARδ as a Novel Target for Covalent Modification by 15-deoxy-Δ12, 14-prostaglandin J₂. ACS Chem. Biol. (2018).

Change, Y.-C., Chen, Y.-J., Huang, C.-W., et al. Cyclic stretch facilitates myogenesis in C2C12 myoblasts and rescues thiazolidinedione-inhibited myotube formation. Front. Bioeng. Biotechnol. 4(27), (2016).

Wang, X., Wang, G., Shi, Y., et al. PPAR-delta promotes survival of breast cancer cells in harsh metabolic conditions. Oncogenesis 5, e232 (2016).