For immunochemical analysis of CD25
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CD25/Interleukin-2Rα Monoclonal Antibody (PE) (Clone 143-13)

Item No. 10339

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Cluster of Differentiation 25
  • IL-2Rα
  • ILR2A
  • Interleukin-2 Receptor Subunit α
  • p55
  • Tac
Clone Designation
143-13
Formulation (Request formulation change)
100 µg of affinity-purified, PE labeled monoclonal antibody
Storage Buffer
TBS, pH 7.4, with 50% glycerol, 5 mg/ml BSA, and 0.02% sodium azide
Host
Mouse
Isotype
IgG1
Applications
(+) FC, ICC, IHC (frozen sections); (−) WB
Species Reactivity
(+) Human CD25
UniProt Accession №
P01589
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    1 ea (100 µg)

    $388

    Add
    Cayman Chemical
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    Product Description

    CD25, also known as interleukin-2 receptor α (IL-2Rα), is a transmembrane glycoprotein.1 It is composed of two domains, D1 and D2, and associates with the CD122/IL-2β and CD132/IL-2γc subunits to form the heterotrimeric IL-2 receptor. CD25 is expressed in activated T and B cells, regulatory T cells, and lymphokine-activated killer (LAK) cells.2,3,4 Activation of IL-2R on T cells increases the expression of CD25 and leads to T cell proliferation and differentiation, as well as activation-induced T cell death.3,4 ILR2A expression and CD25 protein levels in bone marrow are increased in patients with acute myeloid leukemia (AML) and associated with poor prognosis.3 SNPs in ILR2A and elevated serum levels of a soluble form of CD25 are associated with an increased risk of Graves' disease.5 Cayman’s CD25/Interleukin-2Rα Monoclonal Antibody (PE) (Clone 143-13) is composed of a CD25 monoclonal antibody conjugated to phycoerythrin (PE) and can be used for flow cytometry (FC), immunocytochemistry (ICC), and immunohistochemistry (IHC) applications.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Stauber, D.J., Debler, E.W., Horton, P.A., et al. Crystal structure of the IL-2 signaling complex: Paradigm for a heterotrimeric cytokine receptor. Proc. Natl. Acad. Sci. USA 103(8), 2788-2793 (2006).

    2. Volkó, J., Kenesei, Á., Zhang, M., et al. IL-2 receptors preassemble and signal in the ER/Golgi causing resistance to antiproliferative anti-IL-2Rα therapies. Proc. Natl. Acad. Sci. USA 116(42), 21120-21130 (2019).

    3. Li, J., Ran, Q., Xu, B., et al. Role of CD25 expression on prognosis of acute myeloid leukemia: A literature review and meta-analysis. PLoS One 15(7), e0236124 (2020).

    4. Waldmann, T.A. The IL-2/IL-2 receptor system: A target for rational immune intervention. Immunol. Today 14(6), 264-270 (1993).

    5. Lee, H.J., Li, C.W., Hammerstad, S.S., et al. Immunogenetics of autoimmune thyroid diseases: A comprehensive review. J. Autoimmun. 64, 82-90 (2015).