Host: E. coli • AA: 342-460 (partial protein) • Tag: N-terminal GST • MW: 40.6 kDa
Features
  • Due to limited shelf life and/or restricted production, this product is available as custom order only. Please contact our sales office for pricing and lead time.
  • Cayman’s BRD4 bromodomain 2 TR-FRET Assay Kit (Item No. 600530) was developed using BRD4 bromodomain 2 (human recombinant; GST-tagged) protein
  • Bulk sizes available upon request
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BRD4 bromodomain 2 (human, recombinant; GST-tagged)

Item No. 11066

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Bromodomain containing protein 4
  • HUNK1
  • MCAP
Purity
≥80% estimated by SDS-PAGE
Source
Recombinant N-terminal GST-tagged protein expressed in E. coli
Amino Acids
342-460 (partial protein)
MW
40.6 kDa
Formulation (Request formulation change)
50 mM Tris, pH 7.5, with 500 mM sodium chloride and 5% glycerol
UniProt Accession №
O60885
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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    100 µg

    $329

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    1 mg

    $2,303

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    Product Description

    Bromodomain-containing protein 4 (BRD4) is a member of the bromodomain and extra-terminal domain (BET) family.1 It is a ubiquitously expressed nuclear protein with roles in a variety of cellular processes, including regulation of gene transcription, cell cycle progression, and viral genome segregation.1,2 BRD4 is comprised of two N-terminal bromodomains (BD1 and BD2) that can bind to acetylated lysine residues in histones, serving to couple histone acetylation marks to the transcriptional regulation of target promoters, an extra-terminal (ET) domain that facilitates protein-protein interactions, and a C-terminal motif, and can be expressed as either a long or short isoform generated via alternative splicing.1,2,3 In addition to binding acetylated lysine residues on histones, BRD4 can bind to a variety of non-histone proteins and protein complexes, including positive transcription elongation factor b (P-TEFb) and the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) envelope (E) protein, a transmembrane protein involved in CoV virion assembly and pathogenesis of the related virus, SARS-CoV.1,4,5,6 In mice, knock-down of Brd4 is embryonic lethal.2 Chromosomal translocations leading to the fusion of BRD4 with the nuclear protein in testis (NUT) gene and resulting in expression of BRD4-NUT fusion proteins are associated with NUT midline carcinoma (NMC).1,3 Inhibition of BRD4-NUT binding to chromatin by the BET bromodomain inhibitor (+)-JQ1 (Item No. 11187) induces tumor regression and prolongs survival in NMC mouse xenograft models.7 Cayman’s BRD4 bromodomain 2 (human, recombinant; GST-tagged) protein can be used for ELISA, Western blot (WB), and binding assay applications.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Taniguchi, Y. The bromodomain and extra-terminal domain (BET) family: Functional anatomy of BET paralogous proteins. Int. J. Mol. Sci. 17(11), E1849 (2016).

    2. Wu, S.Y., and Chiang, C.M. The double bromodomain-containing chromatin adaptor Brd4 and transcriptional regulation. The Journal of Biological Chemisty 282(18), 13141-13145 (2007).

    3. Wang, C.Y., and Filippakopoulos, P. Beating the odds: BETs in disease. Trends Biochem. Sci. 40(8), 468-479 (2015).

    4. Gordon, D.E., Jang, G.M., Bouhaddou, M., et al. A SARS-CoV-2 protein interaction map reveals targets for drug repurposing. Nature 583(7816), 459-468 (2020).

    5. Kandeel, M., Ibrahim, A., Fayez, M., et al. From SARS and MERS CoVs to SARS-CoV-2: Moving toward more biased codon usage in viral structural and nonstructural genes. J. Med. Virol. 92(6), 660-666 (2020).

    6. Schoeman, D., and Fielding, B.C. Coronavirus envelope protein: Current knowledge. Virol. J. 16(1), 69 (2019).

    7. Goldstein, D.P., and Kosasa, T.S. The subunit radioimmunoassay for human chorionic gonadotropin - clinical applications. Progress in gynecology 145-184 (1975).