Host: E. coli • AA: 65-459 • Tag: N-terminal GST • MW: 71.2 kDa
Features
  • Cayman’s BRD2 bromodomains 1 and 2 TR-FRET Assay Kit (Item No. 600810) was developed using BRD2 bromodomain 1 and 2 (human recombinant) protein.
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BRD2 bromodomains 1 and 2 (human, recombinant)

Item No. 11069

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Bromodomain containing protein 2
  • RING3
  • RNF3
Purity
≥65% estimated by SDS-PAGE
Source
Recombinant GST-tagged protein expressed in E. coli
Amino Acids
65-459
MW
71.2 kDa
Formulation (Request formulation change)
50 mM Tris, pH 7.5, with 500 mM sodium chloride, 5% glycerol, and 5 mM β-mercaptoethanol
UniProt Accession №
P25440
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    100 µg

    $329

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    Product Description

    Bromodomain-containing 2 (BRD2) is a transcriptional regulator that is a member of the bromodomain and extra-terminal (BET) family.1 It is ubiquitously expressed and localizes to the nucleus. BRD2 is composed of two N-terminal bromodomains (BD1 and BD2) that bind acetylated lysine on histones, serving to couple histone acetylation marks to the transcriptional regulation of target promoters, and an extra-terminal domain that mediates chromatin interactions.1,2 BRD2 associates with transcription effector and regulator proteins, including RNA polymerase II, histone acetylases and deacetylases, and transcriptional co-activators and co-repressors to form a transcription complex that regulates the expression of genes involved in inflammation and cell proliferation.1,3 BRD2 also binds to the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) envelope (E) protein, a transmembrane protein involved in CoV virion assembly and pathogenesis of the related virus, SARS-CoV.4,5,6 siRNA knockdown of Brd2 reduces LPS-induced production of TNF and IL-6 in mouse bone marrow-derived macrophages (BMDMs).7 Genetic deletion of Brd2 in mice induces neural tube defects and is embryonic lethal.1 Brd2+/- mice have decreased numbers of GABAergic neurons and a reduced flurothyl-induced seizure threshold. SNPs in BRD2 have been found in individuals with juvenile myoclonic epilepsy. Cayman’s BRD2 bromodomains 1 and 2 (human, recombinant) protein can be used for Western blot (WB) applications.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Taniguchi, Y. The bromodomain and extra-terminal domain (BET) family: Functional anatomy of BET paralogous proteins. Int. J. Mol. Sci. 17(11), E1849 (2016).

    2. Hnilicová, J., Hozeifi, S., Stejskalová, E., et al. The C-terminal domain of Brd2 is important for chromatin interaction and regulation of transcription and alternative splicing. Mol. Biol. Cell 24(22), 3557-3568 (2013).

    3. Gilan, O., Rioja, I., Knezevic, K., et al. Selective targeting of BD1 and BD2 of the BET proteins in cancer and immuno-inflammation. Science 368(6489), 387-394 (2020).

    4. Gordon, D.E., Jang, G.M., Bouhaddou, M., et al. A SARS-CoV-2 protein interaction map reveals targets for drug repurposing. Nature 583(7816), 459-468 (2020).

    5. Kandeel, M., Ibrahim, A., Fayez, M., et al. From SARS and MERS CoVs to SARS-CoV-2: Moving toward more biased codon usage in viral structural and nonstructural genes. J. Med. Virol. 92(6), 660-666 (2020).

    6. Schoeman, D., and Fielding, B.C. Coronavirus envelope protein: Current knowledge. Virol. J. 16(1), 69 (2019).

    7. Belkina, A.C., Nikolajczyk, B.S., and Denis, G.V. BET protein function is required for inflammation: Brd2 genetic disruption and BET inhibitor JQ1 impair mouse macrophage inflammatory responses. J. Immunol. 190(7), 3670-3678 (2013).