MBD2 (human recombinant; methyl binding domain aa 150-220)

DNA methylation occurs mainly at the 5’-position of cytosine rings (5-methylcytosine (5mC)). The 5mC bases are found largely in genomic regions with a high frequency of cytosines and guanines, called CpG islands. Methyl-CpG binding domain 2 (MBD2) protein specifically binds to methylated promoters on CpG islands. The MBD protein family consists of five members: MeCP2, MBD1, MBD2, MBD3, and MBD4.^1,2,3 The sequence similarity between the MBD proteins is limited to the MBD domains.⁴ MBD2 binding to 5mC facilitates the recruitment of chromatin remodeling and transcriptional repressor complexes, which results in a repressive chromatin state.^5,4 MBD2 has been implicated in breast cancer progression. Silencing of MBD2 protein expression was found to be sufficient to initiate and maintain tumor suppressor gene transcription.⁶

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1. Fatemi, M., and Wade, P.A. MBD family proteins: Reading the epigenetic code. J. Cell Sci. 119(Pt. 15), 3033-3037 (2006).

2. Dhasarathy, A., and Wade, P.A. The MBD protein family-reading an epigenetic mark? Mutat. Res. 647(1-2), 39-43 (2008).

3. Hendrich, B., and Bird, A. Identification and characterization of a family of mammalian methyl-CpG binding proteins. Mol. Cell. Biol. 18(11), 6538-6547 (1998).

4. Berger, J., and Bird, A. Role of MBD2 in gene regulation and tumorigenesis. Biochem. Soc. Trans. 33(Pt. 6), 1537-1540 (2005).

5. Ng, H.H., Zhang, Y., Hendrich, B., et al. MBD2 is a transcriptional repressor belonging to the MeCP1 histone deacetylase complex. Nat. Genet. 23(1), 58-61 (1999).

6. Mian, O.Y., Wang, S.Z., Zhu, S.Z., et al. Methyl binding domain protein 2 (MBD2) dependent proliferation and survival of breast cancer cells. Mol. Cancer Res. 9(8), 1152-1162 (2011).