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BRDT bromodomain 2 (human recombinant)

Item No. 11649

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • BRD6
  • Bromodomain testis-specific protein
  • Cancer/testis antigen 9
  • CT9
  • RING3-like protein
Purity
≥90% (estimated by SDS-PAGE)
Source
recombinant N-terminal GST-tagged protein expressed in E. coli
MW
41.2 kDa
Formulation (Request formulation change)
50 mM Tris, pH 7.5, with 500 mM sodium chloride, 5% glycerol, and 5 mM β-mercaptoethanol
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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    100 µg

    $395

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    Product Description

    The acetylation of histone lysine residues plays a crucial role in the epigenetic regulation of gene transcription. Acetylated lysine residues are recognized by a small protein domain known as a bromodomain.1 These domains function in the linking of protein complexes to acetylated nucleosomes, thereby controlling chromatin structure and gene expression. Thus, bromodomains serve as “readers” of histone acetylation marks regulating the transcription of target promoters.2 Bromodomain testis specific (BRDT) shares homology with the RING3 protein. The two bromodomains of BRDT recognize acetylated histone H4. Loss of BRDT leads to defects in spermatogenesis.3 In addition to testis specific expression, BRDT was found in approximately 20% of non-small cell lung cancers.4

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Mujtaba, S., Zeng, L., and Zhou, M.-M. Structure and acetyl-lysine recognition of the bromodomain. Oncogene 26(37), 5521-5527 (2007).

    2. Muller, S., Filippakopoulos, P., and Knapp, S. Bromodomains as therapeutic targets. Expert Rev. Mol. Med. 13, e29 (2011).

    3. Barda, S., Paz, G., Yogev, L., et al. Expression of BET genes in testis of men with different spermatogenic impairments. Fertil. Steril. 97(1), 46-52 (2012).

    4. Scanlan, M.J., Altorki, N.K., Gure, A.O., et al. Expression of cancer-testis antigens in lung cancer: Definition of bromodomain testis-specific gene (BRDT) as a new CT gene, CT9. Cancer Lett. 150(2), 155-164 (2000).

    Product Citations

    Zolotarjova, N.I., and Wynn, R. Binding assays for bromodomain proteins: Their utility in drug discovery in oncology and inflammatory disease. Curr. Protoc. Pharm. 80(1), 3.16.11-13.16.14 (2018).