A RIPK1 inhibitor
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Cl-Necrostatin-1

Item No. 11656

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
5-[(7-chloro-1H-indol-3-yl)methyl]-3-methyl-2-thioxo-4-imidazolidinone
CAS Number
862377-51-3
Synonyms
  • 7-Cl-Nec-1
  • 7-Cl-Necrostatin-1
  • Nec-1f
  • Necrostatin-1f
Molecular Formula
C13H12ClN3OS
Formula Weight
293.8
Purity
≥95%
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMF: 20 mg/mlDMF:PBS (pH 7.2) (1:1): 0.5 mg/mlDMSO: 14 mg/mlEthanol: 3 mg/ml
λmax
221, 267 nm
SMILES
O=C(C(N1)CC2=CNC3=C(Cl)C=CC=C32)N(C)C1=S
InChi Code
InChI=1S/C13H12ClN3OS/c1-17-12(18)10(16-13(17)19)5-7-6-15-11-8(7)3-2-4-9(11)14/h2-4,6,10,15H,5H2,1H3,(H,16,19)
InChi Key
OKOAUGRWEJDVCB-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    1 mg

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    Product Description

    Cl-Necrostatin-1 is an inhibitor of receptor-interacting protein kinase 1 (RIPK1).1 It inhibits RIPK1 activity when used at concentrations of 0.3, 3, and 30 µM. It also inhibits TNF-α-induced necroptosis in Jurkat cells deficient in Fas-associated death domain protein (FADD; EC50 = 180 nM), a modification that prevents caspase activation in response to death-domain receptor signaling.2,3 Cl-Necrostatin-1 (2 µl of a 4 mM solution, i.c.v.) reduces infarct size when administered pre- and post-occlusion, or post-occlusion only, in a mouse model of middle cerebral artery occlusion (MCAO).

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Degterev, A., Hitomi, J., Germscheid, M., et al. Identification of RIP1 kinase as a specific cellular target of necrostatins. Nat. Chem. Biol. 4(5), 313-321 (2008).

    2. Teng, X., Degterev, A., Jagtap, P., et al. Structure-activity relationship study of novel necroptosis inhibitors. Bioorg. Med. Chem. Lett. 15(22), 5039-5044 (2005).

    3. Degterev, A., Huang, Z., Boyce, M., et al. Chemical inhibitor of nonapoptotic cell death with therapeutic potential for ischemic brain injury. Nat. Chem. Biol. 1(2), 112-119 (2005).