A metabolite of vitamin D2
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Labeled Version(s)
3485325-hydroxy Vitamin D2-d6
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25-hydroxy Vitamin D2

Item No. 12078

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
4-methylene-3-[(2E)-2-[(1R,3aS,7aR)-octahydro-1-[(1R,2E,4S)-5-hydroxy-1,4,5-trimethyl-2-hexenyl]-7a-methyl-4H-inden-4-ylidene]ethylidene]-cyclohexanol
CAS Number
21343-40-8
Synonyms
  • Ercalcidiol
  • 25-Hydroxyergocalciferol
  • 25(OH)-Vitamin D2
Molecular Formula
C28H44O2
Formula Weight
412.7
Purity
≥98%
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMF: 20 mg/mlDMSO: 20 mg/mlEthanol: 20 mg/mlEthanol:PBS (pH 7.2)(1:2): 0.3 mg/ml
λmax
211, 264 nm
SMILES
C=C1CC[C@H](O)C/C1=C\C=C2CCC[C@@]3(C)[C@@]\2([H])CC[C@]3([H])[C@H](C)/C=C/[C@H](C)C(C)(O)C
InChi Code
InChI=1S/C28H44O2/c1-19-10-14-24(29)18-23(19)13-12-22-8-7-17-28(6)25(15-16-26(22)28)20(2)9-11-21(3)27(4,5)30/h9,11-13,20-21,24-26,29-30H,1,7-8,10,14-18H2,2-6H3/b11-9+,22-12-,23-13+/t20-,21+,24+,25-,26+,28-/m1/s1
InChi Key
KJKIIUAXZGLUND-UURWAHJLSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    500 µg

    $81

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    1 mg

    $145

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    5 mg

    $475

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    Product Description

    25-hydroxy Vitamin D2 is a metabolite of vitamin D2 (Item No. 11791).1 It is formed from vitamin D2 by the cytochrome P450 (CYP) isoforms CYP2R1 and CYP27A1, which have 25-hydroxylase activity, and is bound to vitamin D binding protein (DBP) and albumin in the liver, then secreted into the bloodstream.2 25-hydroxy Vitamin D2 is transported to the kidney, where it is preferentially 24-hydroxylated by CYP24A1 to produce 24,25-dihydroxy vitamin D2.2 It can also be hydroxylated by CYP27B1 to produce 1,25-dihydroxy vitamin D2.2,1 Serum levels of 25-hydroxy vitamin D2 have been used as a marker of vitamin D status.3

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. DeLuca, H.F., Sicinski, R.P., Tanaka, Y., et al. Biological activity of 1,25-dihydroxyvitamin D2 and 24-epi-1,25-dihydroxyvitamin D2. Am. J. Physiol. 254(4 Pt 1), E402-406 (1998).

    2. Bikle, D. Vitamin D: Production, metabolism, and mechanisms of action. Endotext (2000).

    3. Saenger, A.K., Laha, T.J., Bremner, D.E., et al. Quantification of serum 25-hydroxyvitamin D2 and D3 using HPLC-tandem mass spectrometry and examination of reference intervals for diagnosis of vitamin D deficiency. Am. J. Clin. Pathol. 125(6), 914-920 (2006).