An internal standard for the quantification of 25-hydroxy vitamin D2
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Unlabeled Version(s)
1207825-hydroxy Vitamin D2
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25-hydroxy Vitamin D2-d6

Item No. 34853

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
4-methylene-3-[(2E)-2-[(1R,3aS,7aR)-octahydro-1-[(1R,2E,4S)-5-hydroxy-1,4-dimethyl-5-(methyl-d3)-2-hexen-1-yl-6,6,6-d3]-7a-methyl-4H-inden-4-ylidene]ethylidene]-cyclohexanol
CAS Number
1262843-46-8
Synonyms
  • Ercalcidiol-d6
  • 25-Hydroxyergocalciferol-d6
  • 25(OH)-Vitamin D2-d6
Molecular Formula
C28H38D6O2
Formula Weight
418.7
Purity
≥99% deuterated forms (d1-d6)
Formulation
A solid
Solubility(Learn about Variance in Solubility)
Chloroform: Slightly solubleMethanol: Slightly soluble
SMILES
C=C1CC[C@H](O)C/C1=C/C=C2CCC[C@@]3(C)[C@@]\2([H])CC[C@]3([H])[C@@H](/C=C/[C@H](C)C(C([2H])([2H])[2H])(O)C([2H])([2H])[2H])C
InChi Code
InChI=1S/C28H44O2/c1-19-10-14-24(29)18-23(19)13-12-22-8-7-17-28(6)25(15-16-26(22)28)20(2)9-11-21(3)27(4,5)30/h9,11-13,20-21,24-26,29-30H,1,7-8,10,14-18H2,2-6H3/b11-9+,22-12+,23-13-/t20-,21+,24+,25-,26+,28-/m1/s1/i4D3,5D3
InChi Key
KJKIIUAXZGLUND-SGQRFKNGSA-N
Origin
Synthetic
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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    1 mg

    $1,169

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    Product Description

    25-hydroxy Vitamin D2-d6 is intended for use as an internal standard for the quantification of 25-hydroxy vitamin D2 (Item No. 12078) by GC- or LC-MS. 25-hydroxy Vitamin D2 is a metabolite of vitamin D2 (Item No. 11791).1 It is formed from vitamin D2 by the cytochrome P450 (CYP) isoforms CYP2R1 and CYP27A1, which have 25-hydroxylase activity, and is bound to vitamin D binding protein (DBP) and albumin in the liver, then secreted into the bloodstream.2 25-hydroxy Vitamin D2 is transported to the kidney, where it is preferentially 24-hydroxylated by CYP24A1 to produce 24,25-dihydroxy vitamin D2.2 It can also be hydroxylated by CYP27B1 to produce 1,25-dihydroxy vitamin D2.2,1 Serum levels of 25-hydroxy vitamin D2 have been used as a marker of vitamin D status.3

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. DeLuca, H.F., Sicinski, R.P., Tanaka, Y., et al. Biological activity of 1,25-dihydroxyvitamin D2 and 24-epi-1,25-dihydroxyvitamin D2. Am. J. Physiol. 254(4 Pt 1), E402-406 (1998).

    2. Bikle, D. Vitamin D: Production, metabolism, and mechanisms of action. Endotext (2000).

    3. Saenger, A.K., Laha, T.J., Bremner, D.E., et al. Quantification of serum 25-hydroxyvitamin D2 and D3 using HPLC-tandem mass spectrometry and examination of reference intervals for diagnosis of vitamin D deficiency. Am. J. Clin. Pathol. 125(6), 914-920 (2006).