An inhibitor of autophagy
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3-Methyladenine

Item No. 13242

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
3-methyl-3H-purin-6-amine
CAS Number
5142-23-4
Synonyms
  • 3-MA
  • NSC 66389
Molecular Formula
C6H7N5
Formula Weight
149.2
Purity
≥95%
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMF: 2 mg/mlDMSO: 1.4 mg/mlEthanol: 5 mg/mlPBS (pH 7.2): 2 mg/ml
λmax
212, 274 nm
SMILES
NC(N=CN1C)=C2C1=NC=N2
InChi Code
InChI=1S/C6H7N5/c1-11-3-10-5(7)4-6(11)9-2-8-4/h2-3H,7H2,1H3
InChi Key
FSASIHFSFGAIJM-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    25 mg

    $37

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    50 mg

    $69

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    100 mg

    $128

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    Product Description

    3-Methyladenine (3-MA) is a specific inhibitor of PI3K activity and one of the most widely used inhibitors of the initial phase of the autophagic process: the sequestering of cytoplasmic material by the lysosome.1,2 At 5 mM, 3-MA inhibits protein degradation in rat hepatocytes by 65%.1 3-MA has been shown to block class I, class II, and class III PI3Ks, including some downstream targets, and to suppress the invasion of highly metastatic human fibrosarcoma HT1080 cells at 10 mM.3,4

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Seglen, P.O., and Gordon, P.B. 3-Methyladenine: Specific inhibitor of autophagic/lysosomal protein degradation in isolated rat hepatocytes. Proc. Natl. Acad. Sci. USA 79, 1889-1892 (1982).

    2. Chang, Y.Y., Juhász, G., Goraksha-Hicks, P., et al. Nutrient-dependent regulation of autophagy through the target of rapamycin pathway. Biochem. Soc. Trans. 37, 232-236 (2009).

    3. Wu, Y.T., Tan, H.L., Shui, G., et al. Dual role of 3-methyladenine in modulation of autophagy via different temporal patterns of inhibition on class I and III phosphoinositide 3-kinase. The Journal of Biological Chemisty 285(14), 10850-10861 (2010).

    4. Ito, S., Koshikawa, N., Mochizuki, S., et al. 3-Methyladenine suppresses cell migration and invasion of HT1080 fibrosarcoma cells through inhibiting phosphoinositide 3-kinases independently of autophagy inhibition. Int. J. Oncol. 31, 261-268 (2007).

    Product Citations

    Yuen, C.-K., Wong, W.-M., Mak, L.-F., et al. Suppression of SARS-CoV-2 infection in ex-vivo human lung tissues by targeting class III phosphoinositide 3-kinase. J. Med. Virol. 93(4), 2076-2083 (2021).

    Hu, S., Sechi, M., Singh, P.K., et al. A novel redox modulator induces a GPX4-mediated cell death that is dependent on iron and reactive oxygen species. J. Med. Chem. 63(17), 9838-9855 (2020).

    Sugawara, E., Kato, M., Kudo, Y., et al. Autophagy promotes citrullination of VIM (vimentin) and its interaction with major histocompatibility complex class II in synovial fibroblasts. Autophagy 16(5), 946-955 (2020).

    Liu, A., Guo, E., Yang, J., et al. Young plasma reverses age-dependent alterations in hepatic function through the restoration of autophagy. Aging Cell 17(1), e12708 (2018).

    Chen, J., Wang, Q., Feng, X., et al. Umbilical cord-derived mesenchymal stem cells suppress autophagy of T cells in patients with systemic lupus erythematosus via transfer of mitochondria. Stem Cells Int. 2016, 4062789 (2016).