CFDA-SE

CFDA-SE is a stable, cell-permeable dye that consists of a fluorescein molecule containing two acetate moieties and a succinimidyl ester (SE) functional group.¹ Upon diffusion into the intracellular environment, the acetate groups are cleaved by cellular esterases leaving CFSE (Item No. 16802), which is fluorescent and not cell permeable.² CFSE covalently couples to intracellular molecules via its succinimidyl group and can be retained in cells for at least eight weeks.³ CFDA-SE is often used to assay cell proliferation as it is partitioned with high fidelity between daughter cells for up to eight generational divisions.^1,4,5 CFSE, the cleavage product of CFDA-SE, displays excitation/emission maxima of 491/518 nm, respectively.³

WARNING This product is not for human or veterinary use.

1. Lyons, A.B. Analysing cell division in vivo and in vitro using flow cytometric measurement of CFSE dye dilution. J. Immunol. Methods 243(1-2), 147-154 (2000).

2. Breeuwer, P., Drocourt, J., Rombouts, F.M., et al. A novel method for continuous determination of the intracellular pH in bacteria with the internally conjugated fluorescent probe 5 (and 6-)-carboxyfluorescein succinimidyl ester. Appl. Environ. Microbiol. 62(1), 178-183 (1996).

3. Weston, S.A., and Parish, C.R. New fluorescent dyes for lymphocyte migration studies. Analysis by flow cytometry and fluorescence microscopy. J. Immunol. Methods 133(1), 87-97 (1990).

4. Parish, C.R., Glidden, M.H., Quah, B.J.C., et al. Use of the intracellular fluorescent dye CFSE to monitor lymphocyte migration and proliferation. Curr. Protoc. Immunol. Supp. 84, 4.9.1-4.9.13 (2009).

5. Lecoeur, H., Février, M., Garcia, S., et al. A novel flow cytometric assay for quantitation and multiparametric characterization of cell-mediated cytotoxicity. J. Immunol. Methods 253(1-2), 177-187 (2001).