Pure human recombinant protein
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ATAD2 bromodomain (human recombinant)

Item No. 14490

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • ANCCA
  • ATPase Family AAA Domain-containing Protein 2
Purity
≥90%
Source
recombinant N-terminal GST-tagged protein expressed in E. coli
Amino Acids
981-1,108 (partial protein)
MW
43.1 kDa
Formulation (Request formulation change)
50 mM Tris, pH 8.0, containing 150 mM sodium chloride and 20% glycerol
UniProt Accession №
Q6PL18
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

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    100 µg

    $395

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    Product Description

    The acetylation of histone lysine residues plays a crucial role in the epigenetic regulation of gene transcription. Acetylated lysine residues are recognized by a small protein domain known as a bromodomain.1 These domains function in linking protein complexes to acetylated nucleosomes, thereby controlling chromatin structure and gene expression. Thus, bromodomains serve as "readers" of histone acetylation marks regulating the transcription of target promoters.2 ATAD2 is an AAA+ ATPase-containing nuclear transcriptional coactivator for the estrogen and androgen receptors.3,4 ATAD2 is highly expressed in several types of tumors and has been proposed to link the E2F and MYC signaling pathways. Binding to the MYC oncogene stimulates its transcriptional activity, leading to the development of aggressive cancers with poor prognosis.5 ATAD2 is important for the assembly of chromatin modifying complexes and its bromodomain associates with acetylated lysine 14 on histone H3 to regulate the genes required for cell cycle progression.3,6

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Mujtaba, S., Zeng, L., and Zhou, M.-M. Structure and acetyl-lysine recognition of the bromodomain. Oncogene 26(37), 5521-5527 (2007).

    2. Muller, S., Filippakopoulos, P., and Knapp, S. Bromodomains as therapeutic targets. Expert Rev. Mol. Med. 13, e29 (2011).

    3. Kalashnikova, E.V., Revenko, A.S., Gemo, A.T., et al. ANCCA/ATAD2 overexpression identifies breast cancer patients with poor prognosis, acting to drive proliferation and survival of triple-negative cells through control of B-Myb and EZH2. Cancer Res. 70(22), 9402-9412 (2010).

    4. Zou, J.X., Revenko, A.S., Li, L.B., et al. ANCCA, an estrogen-regulated AAA+ ATPase coactivator for ERα, is required for coregulator occupancy and chromatin modification. Proc. Natl. Acad. Sci. USA 104(46), 18067-18072 (2007).

    5. Ciró, M., Prosperini, E., Quarto, M., et al. ATAD2 is a novel cofactor for MYC, overexpressed and amplified in aggressive tumors. Cancer Res. 69, 8491-8498 (2009).

    6. Revenko, A.S., Kalashnikova, E.V., Gemo, A.T., et al. Chromatin loading of E2F-MLL complex by cancer-associated coregulator ANCCA via reading a specific histone mark. Mol. Cell. Biol. 30(22), 5260-5272 (2010).