Host: E. coli • AA: 882-1,087 (N- and C-terminal truncation) • Tag: N-terminal GST • MW: 51.4 kDa
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  • Due to limited shelf life and/or restricted production, this product is available as custom order only. Please contact our sales office for pricing and lead time.
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TRIM33 PHD and bromodomain (human recombinant)

Item No. 14661

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Ectodermin Homolog
  • E3 Ubiquitin-protein Ligase TRIM33
  • RFG7
  • TIF1-γ
  • Transcriptional Intermediary Factor 1-γ
  • Tripartite Motif Containing 33
Purity
≥80% estimated by SDS-PAGE
Source
Recombinant N-terminal GST-tagged protein expressed in E. coli
Amino Acids
882-1,087 (N- and C-terminal truncation)
MW
51.4 kDa
Formulation (Request formulation change)
50 mM Tris, pH 8.0, with 150 mM sodium chloride and 20% glycerol
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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    1 mg

    $2,195

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    Product Description

    TRIM33 (TIF1-γ) is a multi-domain regulator of transcription. It contains a RING domain with E3 ubiquitin ligase activity, two B-boxes, a coiled-coil domain, a PHD domain, and a bromodomain. TRIM33 is necessary for embryogenesis, and mice homozygous for TRIM33 knock-out do not survive to embryonic day 9.5.1 TRIM33 is targeted to DNA by its tandem PHD and bromodomain which bind histone 3 at trimethylated lysine 9 (H3K9me3) and acetylated lysine 18 (H3K18ac), respectively.2 TRIM33 represses TGF-β signaling by sequestering SMAD2/3, and by ubiquitinating SMAD4.3,4,5 The TRIM33-SMAD2/3 complex upregulates transcription at sites of H3K9 trimethylation. During the DNA damage response, TRIM33 targets Amplified in Liver Cancer 1 (ALC1) to sites of DNA damage.6 Deletion of either the PHD or bromodomain of TRIM33 prevents localization of TRIM33 to sites of DNA damage.6 This protein product contains the PHD and bromodomains of TRIM33.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Kim, J., and Kaartinen, V. Generation of mice with a conditional allele for Trim33. Genesis 46(6), 329-333 (2008).

    2. Xi, Q., Wang, Z., Zaromytidou, A.I., et al. A poised chromatin platform for TGF-β access to master regulators. Cell 147(7), 1511-1524 (2011).

    3. Fattet, T., Ay, A., Bonneau, B., et al. TIF1γ requires sumoylation to exert its repressive activity on TGFβ signaling. J. Cell Sci. 126(16), 3713-3723 (2013).

    4. Dupont, S., Mamidi, A., Cordenonsi, M., et al. FAM/USP9x, a deubiquitinating enzyme essential for TGFβ signaling, controls Smad4 monoubiquitination. Cell 136(1), 123-135 (2009).

    5. Agricola, E., Randall, R.A., Gaarenstroom, T., et al. Recruitment of TIF1γ to chromatin via its PHD finger-bromodomain activates its ubiquitin ligase and transcriptional repressor activities. Mol. Cell 43(1), 85-96 (2011).

    6. Kulkarni, A., Oza, J., Yao, M., et al. Tripartite motif-containing 33 (TRIM33) functions in the poly(ADP-ribose)polymerase (PARP)-dependent DNA damage response through interaction with amplified in liver cancer 1 (ALC1). The Journal of Biological Chemisty (2013).