Pure human recombinant protein
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Product Categories

Product Type

Proteins

Research Area

Epigenetics, Transcription, & Translation
Readers
Chromodomains

Application

CBX2 chromodomain (human recombinant)

Item No. 14769

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • CDCA6
  • Cell Division Cycle Associated 6
  • Chromobox Protein Homolog 2 (Drosophila Pc Class)
  • M33
  • MGC10561
  • Modifier 3
  • SRXY5
Purity
≥90% estimated by SDS-PAGE
Source
Recombinant N-terminal GST-tagged protein expressed in E. coli
Amino Acids
2-90
MW
38.2 kDa
Formulation (Request formulation change)
50 mM TRIS, pH 8.0, containing 150 mM sodium chloride and 20% glycerol
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    100 µg

    $382

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    Product Description

    CBX2 is a component of the polycomb repressive complex 1 and represses protein expression.1 The chromodomain regions of CBX2 recognizes histone H3 trimethyl lysine 9 (H3K9me3) or trimethyl lysine 27 (H3K27me3).2,3,4 CBX2 knock-out mice have impaired spleen formation, T cell expansion, and inactivation of the X chromosome, which can be lethal in some cases.5 This protein product contains the chromodomain region of CBX2.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Schoorlemmer, J., Marcos-Gutiérrez, C., Were, F., et al. Ring1A is a transcriptional repressor that interacts with the Polycomb-M33 protein and is expressed at rhombomere boundaries in the mouse hindbrain. EMBO J. 16(19), 5930-5942 (1997).

    2. Nielsen, A.L., Oulad-Abdelghani, M., Ortiz, J.A., et al. Heterochromatin formation in mammalian cells: Interaction between histones and HP1 proteins. Mol. Cell 7(4), 729-739 (2001).

    3. Bernstein, E., Duncan, E.M., Masui, O., et al. Mouse polycomb proteins bind differentially to methylated histone H3 and RNA and are enriched in facultative heterochromatin. Mol. Cell Biol. 26(7), 2560-2569 (2006).

    4. Kaustov, L., Ouyang, H., Amaya, M., et al. Recognition and specificity determinants of the human Cbx chromodomains. The Journal of Biological Chemisty 286(1), 521-529 (2011).

    5. Katoh-Fukui, Y., Owaki, A., Toyama, Y., et al. Mouse polycomb M33 is required for splenic vascular and adrenal gland formation through regulating Ad4BP/SF1 expression. Blood 106(5), 1612-1620 (2005).