Pure human recombinant protein
Features
  • Bulk sizes available upon request
Technical Support & Resources

Visit our FAQ

Contact Us

Toll Free Phone (USA and Canada Only): (888) 526-5351
Direct Phone: (734) 975-3888

Request Technical Support

Technical Support Request

To streamline the process attach the appropriate questionnaire to your inquiry.

Download IHC QuestionnaireDownload WB Questionnaire

View Our Privacy Statement for details on how we use and protect your data. In addition, this site is protected by hCaptcha and its Privacy Policy and Terms of Service apply.

Product Categories

Product Type

Proteins

Research Area

Epigenetics, Transcription, & Translation
Readers
MBT Domains

Application

L3MBTL1 MBT domains (human recombinant; GST-tagged)

Item No. 14775

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • H-l(3)MBT
  • Lethal(3)Malignant Brain Tumor-like Protein 1
  • L(3)MBT-like
Purity
≥90% estimated by SDS-PAGE
Source
Recombinant N-terminal GST-tagged protein expressed in E. coli
Amino Acids
191-350
MW
65.5 kDa
Formulation (Request formulation change)
50 mM Tris, pH 8.0, with 150 mM sodium chloride and 20% glycerol
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
Recommended Products

Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    100 µg

    $382

    Add
    Product Description

    The malignant brain tumor (MBT) domain is structurally related to chromatin binding domains, such as chromodomains, tudor domains, and PWWP-domains.1,2 MBT domain-containing proteins have a variable number of MBT repeats. The MBT domains recognize methylated lysines on histone tails with varying degree of specificity for the various methyl marks.1 L3MBTL1, a human homolog of the Drosophila lethal(3)MBT protein, is a member of the polycomb group (PcG) of proteins that functions as a transcriptional repressor.3 L3MBTL1 contains three MBT repeat domains which collectively bind to either histone H3 or H4.1,4 The second and third MBT domains were found to bind preferentially to mono- and dimethylated lysines of histone H3 at lysine 4 (H3K4me1) and histone H4 at lysine 20 (H4K20me2).4,5 Recognition of methyl-lysine marks by MBT domains leads to chromatin compaction and a repressed transcriptional state.4,6 Further, L3MBTL1 has a tumor suppressor function and is thought to play a role in maintaining genomic stability and DNA replication.7,8 This protein product contains the MBT repeat region of L3MBTL1.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Trojer, P., and Reinberg, D. Beyond histone methyl-lysine binding: How malignant brain tumor (MBT) protein L3MBTL1 impacts chromatin structure. Cell Cycle 7(5), 578-585 (2008).

    2. Maurer-Stroh, S., Dickens, N.J., Hughes-Davies, L., et al. The Tudor domain 'Royal Family': Tudor, plant Agenet, Chromo, PWWP and MBT domains. Trends Biochem. Sci. 28(2), 69-74 (2003).

    3. Boccuni, P., MacGrogan, D., Scandura, J.M., et al. The human L(3)MBT polycomb group protein is a transcriptional repressor and interacts physically and functionally with TEL (ETV6). The Journal of Biological Chemisty 278(17), 15412-15420 (2003).

    4. Trojer, P., Li, G., Sims, R.J., III, et al. L3MBTL1, a histone-methylation-dependent chromatin lock. Cell 129(5), 915-928 (2007).

    5. Kim, J., Daniel, J., Espejo, A., et al. Tudor, MBT and chromo domains gauge the degree of lysine methylation. EMBO Rep. 7(4), 397-403 (2006).

    6. Kalakonda, N., Fischle, W., Boccuni, P., et al. Histone H4 lysine 20 monomethylation promotes transcriptional repression by L3MBTL1. Oncogene 27(31), 4293-4304 (2008).

    7. Acs, K., Luijsterburg, M.S., Ackermann, L., et al. The AAA-ATPase VCP/p97 promotes 53BP1 recruitment by removing L3MBTL1 from DNA double-strand breaks. Nat. Struct. Mol. Biol. 18(12), 1345-1350 (2011).

    8. Gurvich, N., Perna, F., Farina, A., et al. L3MBTL1 polycomb protein, a candidate tumor suppressor in del(20q12) myeloid disorders, is essential for genome stability. Proc. Natl. Acad. Sci. USA 107(52), 22552-22557 (2010).