A fluorogenic CYP1A1/2 substrate
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Methoxyresorufin

Item No. 14852

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
7-methoxy-3H-phenoxazin-3-one
CAS Number
5725-89-3
Synonyms
  • Resorufin methyl ether
Molecular Formula
C13H9NO3
Formula Weight
227.2
Purity
≥90%
Emission
580 nm
Excitation
570 nm
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
DMSO: soluble
SMILES
O=C1C=CC2=NC3=C(C=C(OC)C=C3)OC2=C1
InChi Code
InChI=1S/C13H9NO3/c1-16-9-3-5-11-13(7-9)17-12-6-8(15)2-4-10(12)14-11/h2-7H,1H3
InChi Key
KNYYMGDYROYBRE-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    1 mg

    $69

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    5 mg

    $263

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    Product Description

    Methoxyresorufin is a fluorogenic substrate for the cytochrome P450 (CYP) isoforms CYP1A1 and CYP1A2.1,2,3 Methyoxyresorufin is O-demethylated by CYP1A1/2, releasing resorufin, which displays excitation/emission maxima of 570/580 nm, respectively.1,2,3,4 The appearance of resorufin fluorescence can be used to quantify CYP1A1/2 activity.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Mayer, R.T., Jermyn, J.W., Burke, M.D., et al. Methoxyresorufin as a substrate for the fluorometric assay of insect microsomal O-dealkylases. Pestic. Biochem. Physiol. 7(4), 349-354 (1977).

    2. Nerurkar, P.V., Park, S.S., Thomas, P.E., et al. Methoxyresorufin and benzyloxyresorufin: Substrates preferentially metabolized by cytochromes P4501A2 and 2B, respectively, in the rat and mouse. Biochem. Pharmacol. 46(5), 933-943 (1993).

    3. Caballero, M.V., Ares, I., Martínez, M., et al. Fipronil induces CYP isoforms in rats. Food Chem. Toxicol. 83, 215-221 (2015).

    4. Hofmann, J., and Sernetz, M. Immobilized enzyme kinetics analyzed by flow-through microfluorimetry: Resorufin-β-D-galactopyranoside as a new fluorogenic substrate for β-galactosidase. Anal. Chim. Acta 163, 67-72 (1984).