Host: E. coli • AA: 2-434 • Tag: N-terminal GST • MW: 75 kDa
Features
  • Due to limited shelf life and/or restricted production, this product is available as custom order only. Please contact our sales office for pricing and lead time.
  • Cayman’s BRD3 bromodomains 1 and 2 TR-FRET Assay Kit (Item No. 600820) was developed using BRD3 bromodomain 1 and 2 (human, recombinant) protein
  • Bulk sizes available upon request
Related Products
Alternative(s)
14658BRD3 bromodomain 2 (human, recombinant)
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BRD3 bromodomains 1 and 2 (human, recombinant)

Item No. 14864

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Bromodomain containing protein 3
  • ORFX
  • RING3L
  • RING3-like protein
Purity
≥85% (estimated by SDS-PAGE)
Source
Recombinant human N-terminal GST-tagged BRD3 bromodomains 1 and 2 expressed in E. coli
Amino Acids
2-434
MW
75 kDa
Formulation (Request formulation change)
50 mM Tris, pH 8.0, with 150 mM sodium chloride and 20% glycerol
Storage Buffer
50 mM Tris, pH 8.0, with 150 mM sodium chloride and 20% glycerol
Applications
WB
UniProt Accession №
Q15059
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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    100 µg

    $382

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    Product Description

    Bromodomain-containing protein 3 (BRD3) is a member of the bromodomain and extra-terminal domain (BET) family.1 It is composed of two N-terminal bromodomains (BD1 and BD2) that bind acetylated lysine on histones, serving to couple histone acetylation marks to the transcriptional regulation of target promoters, and an extra-terminal domain that mediates chromatin interactions. BRD3 is ubiquitously expressed and localizes to the nucleus where it preferentially binds acetylated histone H4 lysine 5 (H4K5), H4K12, and H4K14 at active transcription sites.2,3 Knockdown of Brd3 enhances differentiation of C2C12 skeletal muscle myoblasts.4 Chromosomal translocations leading to the fusion of BRD3 with the nuclear protein in testis (NUT) gene and resulting in expression of BRD3-NUT fusion proteins are associated with NUT midline carcinoma (NMC).5 Cayman’s BRD3 bromodomains 1 and 2 protein can be used for Western blot (WB).

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Weidner-Glunde, M., Ottinger, M., and Schulz, T.F. WHAT do viruses BET on? Front. Biosci. (Landmark Ed.) 15, 537-549 (2010).

    2. Thorpe, K.L., Gorman, P., Thomas, C., et al. Chromosomal localization, gene structure and transcription pattern of the ORFX gene, a homologue of the MHC-linked RING3 gene. Gene 200(1-2), 177-183 (1997).

    3. LeRoy, G., Rickards, B., and Flint, S.J. The double bromodomain proteins Brd2 and Brd3 couple histone acetylation to transcription. Mol. Cell 30(1), 51-60 (2008).

    4. Roberts, T.C., Etxaniz, U., Dall'Agnese, A., et al. BRD3 and BRD4 BET bromodomain proteins differentially regulate skeletal myogenesis. Sci. Rep. 7(1), 6153 (2017).

    5. French, C.A., Ramirez, C.L., Kolmakova, J., et al. BRD-NUT oncoproteins: A family of closely related nuclear proteins that block epithelial differentiation and maintain the growth of carcinoma cells. Oncogene 327(15), 2237-2242 (2008).