A fluorescent probe
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Calcein AM

Item No. 14948

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
N-[2-[(acetyloxy)methoxy]-2-oxoethyl]-N-[[3′,6′-bis(acetyloxy)-5′-[[bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]methyl]-3-oxospiro[isobenzofuran-1(3H),9′-[9H]xanthen]-4′-yl]methyl]-glycine, (acetyloxy)methyl ester
CAS Number
890090-35-4
Synonyms
  • Calcein Acetoxymethyl ester
Molecular Formula
C46H46N2O23
Formula Weight
994.9
Purity
≥90%
Emission
517 nm
Excitation
494 nm
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMF: 10 mg/mlDMSO: 10 mg/mlEthanol: 10 mg/mlMethanol: 10 mg/ml
SMILES
O=C1OC2(C3=C1C=CC=C3)C4=CC=C(C(CN(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)=C4OC5=C(CN(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)C(OC(C)=O)=CC=C52)OC(C)=O
InChi Code
InChI=1S/C46H46N2O23/c1-25(49)60-21-64-39(55)17-47(18-40(56)65-22-61-26(2)50)15-32-37(68-29(5)53)13-11-35-43(32)70-44-33(16-48(19-41(57)66-23-62-27(3)51)20-42(58)67-24-63-28(4)52)38(69-30(6)54)14-12-36(44)46(35)34-10-8-7-9-31(34)45(59)71-46/h7-14H,15-24H2,1-6H3
InChi Key
XKFSBWQWNMZWFA-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    500 µg

    $86

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    1 mg

    $163

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    Product Description

    Calcein AM is a cell-permeable dye that, upon entering live cells, is cleaved by intracellular esterases, leaving membrane-impermeant calcein (Item No. 16221), a fluorescent indicator with absorption and emission maxima of 494 and 517 nm, respectively. It can be used as an indicator of cell viability, cell-cell communication, cytotoxicity, or changes in intracellular calcium, fluoride, iron, or mercury.1,2 As calcein is a substrate for multidrug resistance-associated protein 1 (MRP1) and multidrug resistance protein 3 (MDR3, P-glycoprotein 3), calcein AM is used to study these proteins and their regulation.3,4,5

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Hynes, J., Floyd, S., Soini, A.E., et al. Fluorescence-based cell viability screening assays using water-soluble oxygen probes. J. Biomol. Screen. 8, 264-272 (2003).

    2. Sabnis, R.W. Handbook of biological dyes and stains: Synthesis and industrial applications. (2010).

    3. Polli, J.W., Wring, S.A., Humphreys, J.E., et al. Rational use of in vitro P-glycoprotein assays in drug discovery. J. Pharmacol. Exp. Ther. 299(2), 620-628 (2001).

    4. Tiberghien, F., and Loor, F. Ranking of P-glycoprotein substates and inhibitors by a calcein-AM fluorometry screening assay. Anticancer Drugs 7(5), 568-578 (1996).

    5. Szakács, G., Jakab, K., Antal, F., et al. Diagnostics of multidrug resistance in cancer. Pathol.Oncol.Res. 4(4), 251-257 (2016).