A fluorogenic substrate for thioredoxin
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Eosin-Labeled Insulin

Item No. 15495

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Emission
545 nm
Excitation
520 nm
Formulation
Lyophilized fluorescent substrate
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    1 ea

    $168

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    Product Description

    Eosin-labeled insulin is a fluorogenic substrate for thioredoxin.1,2 Upon reduction by thioredoxin, eosin is released and its fluorescence can be used to quantify thioredoxin activity. Eosin displays excitation/emission maxima of 520/545 nm, respectively. This product is also available as part of Cayman’s Thioredoxin Fluorometric Assay Kit (Item No. 500228).

    Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Montano, S.J., Lu, J., Gustafsson, T.N., et al. Activity assays of mammalian thioredoxin and thioredoxin reductase: Fluorescent disulfide substrates, mechanisms, and use with tissue samples. Anal. Biochem. 449, 139-146 (2014).

    2. Yarana, C., Thompson, H., Chaiswing, L., et al. Extracellular vesicle-mediated macrophage activation: An insight into the mechanism of thioredoxin-mediated immune activation. Redox Biol. 26, 101237 (2019).